Product Info Summary
| SKU: | PA2009 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TRAF4 Antibody Picoband®
SKU/Catalog Number
PA2009
BA3003-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF4 Antibody catalog # PA2009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2009)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2009 is reactive to TRAF4 in Human, Mouse, Rat
Observed Molecular Weight
56 kDa
Calculated molecular weight
53.5 kDa
Background of TRAF4
TRAF4 (TNF Receptor-Associated Factor 4), also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF3 gene. TRAF4 is a member of the TNF receptor associated factor (TRAF) family, a family of scaffold proteins. Tomasetto et al. (1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al. (2002) generated Traf4-deficient mice by gene disruption.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2009 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Thymus Tissue, HELA Cell, JURKAT Cell, HEPA Cell,
IHC: Rat Lymphonodus Tissue, Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-TRAF4 antibody, PA2009, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HEPA Cell Lysate
Click image to see more details
Anti-TRAF4 antibody, PA2009, IHC(P)
IHC(P): Rat Lymphonodus Tissue
Click image to see more details
Anti-TRAF4 antibody, PA2009, IHC(P)
IHC(P): Human Lung Cancer Tissue
Specific Publications For Anti-TRAF4 Antibody Picoband® (PA2009)
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1 Customer Q&As for Anti-TRAF4 Antibody Picoband®
Question
We are currently using anti-TRAF4 antibody PA2009 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
B. Zhang
Verified customer
Asked: 2016-02-26
Answer
The anti-TRAF4 antibody (PA2009) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-26


