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Product Info Summary
| SKU: | M03069 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TRAF4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03069
BM4134 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRAF4 Rabbit Monoclonal Antibody catalog # M03069. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03069)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BGH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRAF4
Reactive Species
M03069 is reactive to TRAF4 in Human, Mouse
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03069 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of TRAF4 expression in HeLa cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-TRAF4 Rabbit Monoclonal Antibody (M03069)
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Customer Q&As
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5 Customer Q&As for Anti-TRAF4 Rabbit Monoclonal Antibody
Question
Why can't we get a signal when using M03069 on HELA and liver tissue WB?
Verified customer
Asked: 2023-01-06
Answer
Please see the following tips to improve test result using the Anti-TRAF4 Rabbit Monoclonal Antibody (M03069): 1. Please use lysis buffer with 1%SDS if the LDS lysis buffer is a non-reducing buffer. 2. The membrane cutting area should preferably be within 20-80kDa. Place 50kDa in the middle of PVDF membrane. 3. Load equal amounts (20-30 ug) of protein into each well of the SDS-PAGE gel, along with appropriate molecular weight marker. 1 ug is too little. 4. Block the membrane for 1 hour at room temperature using 1%BSA/TBST or 2-3% non-fat Milk/ TBST. Dilute the antibodies for 1 hour at room temperature using 1%BSA/TBST or 2-3% non-fat Milk/ TBST. 5. Increase the concentration of the primary antibody and the secondary antibody.e.g. primary antibody 1:500-1000, secondary antibody 1:1000-2000. 6. Do not overuse ECL substrate. After substrate incubation, remove the excess ECL substrate from the membrane before exposure (place the membrane vertically, and the ECL substrate liquid does not flow down, and then use paper to absorb the excess ECL substrate around the membrane). This can prevent the flow of ECL substrate from causing strong non-specific bands, dark background and irregular black marks.
Boster Scientific Support
Answered: 2023-01-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-TRAF4 Rabbit Monoclonal antibody M03069. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-28
Question
Is this M03069 anti-TRAF4 Rabbit Monoclonal antibody reactive to the isotypes of TRAF4?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
The immunogen of M03069 anti-TRAF4 Rabbit Monoclonal antibody is A synthesized peptide derived from human TRAF4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-16
Question
Would anti-TRAF4 Rabbit Monoclonal antibody M03069 work for WB with lung?
J. Walker
Verified customer
Asked: 2018-02-09
Answer
According to the expression profile of lung, TRAF4 is highly expressed in lung. So, it is likely that anti-TRAF4 Rabbit Monoclonal antibody M03069 will work for WB with lung.
Boster Scientific Support
Answered: 2018-02-09
Question
I was wanting to use your anti-TRAF4 Rabbit Monoclonal antibody for WB for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
D. Banerjee
Verified customer
Asked: 2015-10-05
Answer
It shows on the product datasheet, M03069 anti-TRAF4 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-10-05
