Anti-TRNAU1AP Antibody Picoband®

Western blot analysis of TRNAU1AP using anti-TRNAU1AP antibody (A15424-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human COLO320 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNAU1AP antigen affinity purified polyclonal antibody (A15424-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRNAU1AP at approximately 38 kDa. The expected band size for TRNAU1AP is at 32 kDa.

Immunoprecipitating TRNAU1AP in Jurkat whole cell lysate.
Western blot analysis of TRNAU1AP using anti-TRNAU1AP antibody (A15424-1);
Lane 1: Jurkat whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-TRNAU1AP antibody in Jurkat whole cell lysate;
Lane 3: anti-TRNAU1AP antibody (2μg) + Jurkat whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRNAU1AP antigen affinity purified polyclonal antibody (A15424-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for TRNAU1AP at approximately 38 kDa. The expected band size for TRNAU1AP is at 32 kDa.

Flow Cytometry analysis of Jurkat cells using anti-TRNAU1AP antibody (A15424-1).
Overlay histogram showing Jurkat cells stained with A15424-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRNAU1AP Antibody (A15424-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.