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Product Info Summary
| SKU: | A15424-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TRNAU1AP Antibody Picoband®
SKU/Catalog Number
A15424-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRNAU1AP Antibody Picoband® catalog # A15424-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRNAU1AP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15424-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRNAU1AP recombinant protein (Position: R21-E282).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15424-1 is reactive to TRNAU1AP in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
32.5 kDa
Background of TRNAU1AP
Enables RNA binding activity. Predicted to be involved in selenocysteine incorporation. Predicted to be located in cytoplasm. Predicted to be active in nucleus. TRNAU1AP binds selenocysteine tRNA (tRNA-sec; see 165060) and is a component of the selenocysteine biosynthesis pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15424-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Jurkat whole cell, human A549 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of TRNAU1AP using anti-TRNAU1AP antibody (A15424-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNAU1AP antigen affinity purified polyclonal antibody (Catalog # A15424-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNAU1AP at approximately 40 kDa. The expected band size for TRNAU1AP is at 32 kDa.
Specific Publications For Anti-TRNAU1AP Antibody Picoband® (A15424-1)
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