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- Table of Contents
TRNAU1AP antibody (A15424-1) performs well in ELISA, Flow Cytometry, IP, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Jurkat cells using anti-TRNAU1AP antibody (A15424-1).
Overlay histogram showing Jurkat cells stained with A15424-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRNAU1AP Antibody (A15424-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Jurkat cells stained with A15424-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRNAU1AP Antibody (A15424-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TRNAU1AP Antibody Picoband®
TRNAU1AP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, WB |
Tested Applications
TRNAU1AP antibody was positively detected by WB in:
human A549 whole cell, human Jurkat whole cell, human MCF-7 whole cell, human COLO320 whole cell, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TRNAU1AP antibody was positively detected by IP in:
Jurkat cell
TRNAU1AP antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-TRNAU1AP Antibody Picoband®
View all TRNAU1AP Antibodies
View all TRNAU1AP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRNAU1AP recombinant protein (Position: R21-E282).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
32.5 kDa
Background of TRNAU1AP
Enables RNA binding activity. Predicted to be involved in selenocysteine incorporation. Predicted to be located in cytoplasm. Predicted to be active in nucleus. TRNAU1AP binds selenocysteine tRNA (tRNA-sec; see 165060) and is a component of the selenocysteine biosynthesis pathway.
Cite This Product
Anti-TRNAU1AP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15424-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rat
99%
High Homology
Sheep
99%
High Homology
Mouse
99%
High Homology
Rabbit
82%
Moderate Homology
Zebrafish
57%
Low homology
Drosophila
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TRNAU1AP antibody
Immunoprecipitation (IP) protocol for TRNAU1AP antibody
Flow Cytometry (FC) protocol for TRNAU1AP antibody
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Product Promise
We promise Anti-TRNAU1AP Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A15424-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026