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- Table of Contents
PECAM1 antibody (M01513-4) performs well in IHC, IHC-F, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
CD31/PECAM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
CD31/PECAM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)
PECAM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IHC, IHC-F, WB |
Tested Applications
PECAM1 antibody was positively detected by WB in:
human Jurkat whole cell,, human placenta tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PECAM1 antibody was positively detected by IHC in:
human lung cancer tissue, human lung cancer tissue, human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PECAM1 antibody was positively detected by IHC-F in:
human placenta tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)
View all PECAM1 Antibodies
View all PECAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2D4
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
82.5 kDa
Background of PECAM1
CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.
Cite This Product
Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
92%
High Homology
Dog
76%
Moderate Homology
Rabbit
74%
Moderate Homology
Cow
72%
Moderate Homology
Sheep
71%
Moderate Homology
Rat
67%
Low homology
Mouse
65%
Low homology
Zebrafish
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PECAM1 antibody
Immunohistochemistry (IHC) protocol for PECAM1 antibody
Publications
Application:
Species:
Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice
Published: 2009-03-01
Journal: CANCER BIOLOGY & THERAPY
Application: WB
Impact Factor: 2.76
Species: Mouse
PMID: 19223770
Review
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1 Review For Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)
Immunohistochemistry for Anti-CD31/PECAM1 Antibody
| SKU | M01513-4 |
|---|---|
| Application | Immunohistochemistry (Paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Lung |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Z. Edwards
Verified customer
Submitted 2020-08-06
Product Promise
We promise Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) performs excellently in relevant applications for IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01513-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026