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Product Info Summary
| SKU: | M01513-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)
SKU/Catalog Number
M01513-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) catalog # M01513-4. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-4)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2D4
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01513-4 is reactive to PECAM1 in Human
Observed Molecular Weight
130 kDa
Calculated molecular weight
82.5 kDa
Background of PECAM1
CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01513-4 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Positive Control
WB: human Jurkat whole cell,, human placenta tissue
IHC: human lung cancer tissue, human lung cancer tissue, human mammary cancer tissue
IHC-F: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates;
Lane 2: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD31/PECAM1 antigen affinity purified monoclonal antibody (Catalog # M01513-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 130KD. The expected band size for CD31/PECAM1 is at 130KD.
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IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
CD31/PECAM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
CD31/PECAM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
CD31/PECAM1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
CD31/PECAM1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) (M01513-4)
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Customer Reviews
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1 Reviews For Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4)
Immunohistochemistry for Anti-CD31/PECAM1 Antibody
Excellent
| SKU | M01513-4 |
|---|---|
| Application | Immunohistochemistry (Paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Lung |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Z. Edwards
Verified customer
Submitted 2020-08-06
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