Anti-CD31/Pecam1 Antibody Picoband™

Boster Bio Anti-CD31/Pecam1 Antibody Picoband™ catalog # A01513-1. Tested in ELISA, WB applications. This antibody reacts with Mouse. Cited in 18 publication(s).

Product Info Summary

SKU: A01513-1
Size: 100μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-CD31/Pecam1 Antibody Picoband™

See all CD31/PECAM-1 products

SKU/Catalog Number

A01513-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD31/Pecam1 Antibody Picoband™ catalog # A01513-1. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD31/Pecam1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01513-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse CD31 recombinant protein (Position: R58-Q273).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01513-1 is reactive to Pecam1 in Mouse

Applications

A01513-1 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Pecam1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08481

Gene ID

18613

Gene Name

Pecam1

Full Name

Platelet endothelial cell adhesion molecule

Weight

81.263kDa

Alternative Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule Pecam1|C85791, Cd31, PECAM-1, Pecam|platelet/endothelial cell adhesion molecule 1|platelet endothelial cell adhesion molecule

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pecam1, check out the Pecam1 Infographic

Pecam1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Pecam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01513-1 has been cited in 18 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shen Song,Zhao Liu,Mohamed Aamer Abubaker,Ling Ding,Ji Zhang,Shengrong Yang,Zengjie Fan,Antibacterial polyvinyl alcohol/bacterial cellulose/ nano-silver hydrogels that effectively promote wound healing,Materials Science and Engineering: C,Volume 126,2021,112171,ISSN 0928-4931,https://doi.org/10.1016/j.msec.2021.112171.
Species: Mouse
A01513-1 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:NA

Wang L,Wu H,Xiong L,Liu X,Yang N,Luo L,Qin T,Zhu X,Shen Z,Jing H,Chen J.Quercetin Downregulates Cyclooxygenase-2 Expression and HIF-1α/VEGF Signaling-Related Angiogenesis in a Mouse Model of Abdominal Aortic Aneurysm.Biomed Res Int.2020 Aug 22;2020:9485398.doi:10.1155/2020/9485398.PMID:32908926;PMCID:PMC7463408.
Species: Mouse
A01513-1 usage in article: APP:IHC, SAMPLE:ABDOMINAL AORTA TISSUE, DILUTION:1:100

MicroRNA-210 promotes angiogenesis in acute myocardial infarction

Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase

The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

WSS25 Inhibits Growth of Xenografted Hepatocellular Cancer Cells in Nude Mice by Disrupting Angiogenesis via Blocking Bone Morphogenetic Protein (BMP)/Smad/Id1 Signaling

Chen X, Zhang M, Chen S, Wang X, Tian Z, Chen Y, Xu P, Zhang L, Zhang L, Zhang L. Cell Transplant. 2017 Aug;26(8):1331-1340. doi: 10.1177/0963689717721216. Peptide-Modified Chitosan Hydrogels Accelerate Skin Wound Healing by Promoting Fibroblast P...

Chen L, Chan Th, Yuan Yf, Hu L, Huang J, Ma S, Wang J, Dong Ss, Tang Kh, Xie D, Li Y, Guan Xy. J Clin Invest. 2010 Apr;120(4):1178-91. Doi: 10.1172/Jci40665. Epub 2010 Mar 24. Chd1L Promotes Hepatocellular Carcinoma Progression And Metastasis In M...

Qiu H, Yang B, Pei Zc, Zhang Z, Ding K. J Biol Chem. 2010 Oct 15;285(42):32638-46. Doi: 10.1074/Jbc.M110.105544. Epub 2010 Aug 2. Wss25 Inhibits Growth Of Xenografted Hepatocellular Cancer Cells In Nude Mice By Disrupting Angiogenesis Via Blocking...

Zhou S, Yi T, Liu R, Bian C, Qi X, He X, Wang K, Li J, Zhao X, Huang C, Wei Y. Mol Cell Proteomics. 2012 Jul;11(7):M112.017988. Doi: 10.1074/Mcp.M112.017988. Epub 2012 Apr 9. Proteomics Identification Of Annexin A2 As A Key Mediator In The Metasta...

Xiu J, Fan J, Li J, Cui G, Lei W. Biomed Res Int. 2014;2014:785146. Doi: 10.1155/2014/785146. Epub 2014 Jun 10. Different Angiogenic Abilities Of Self-Setting Calcium Phosphate Cement Scaffolds Consisting Of Different Proportions Of Fibrin Glue.

Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.

Wu Q, Chang Y, Zhang L, Zhang Y, Tian T, Feng G, Zhou S, Zheng Q, Han F, Huang F. J Cancer. 2013 Nov 21;4(9):727-35. Doi: 10.7150/Jca.7576. Ecollection 2013. Srpk1 Dissimilarly Impacts On The Growth, Metastasis, Chemosensitivity And Angiogenesis O...

Chen J, Jia Zy, Ma Zl, Wang Yy, Teng Gj. Plos One. 2011;6(6):E20790. Doi: 10.1371/Journal.Pone.0020790. Epub 2011 Jun 24. In Vivo Serial Mr Imaging Of Magnetically Labeled Endothelial Progenitor Cells Homing To The Endothelium Injured Artery In Mice.

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16 Customer Q&As for Anti-CD31/Pecam1 Antibody Picoband™

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD31/Pecam1 antibody A01513-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-06

Question

Is there a BSA free version of anti-CD31/Pecam1 antibody A01513-1 available?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD31/Pecam1 antibody A01513-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-29

Question

We are currently using anti-CD31/Pecam1 antibody A01513-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

The anti-CD31/Pecam1 antibody (A01513-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-15

Question

My question regarding product A01513-1, anti-CD31/Pecam1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01513-1 anti-CD31/Pecam1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-09

Question

I see that the anti-CD31/Pecam1 antibody A01513-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-06

Question

I am looking for using your anti-CD31/Pecam1 antibody for r-mmu-210990; pecam1 interactions studies. Has this antibody been tested with western blotting on mouse ovary tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

I appreciate your inquiry. This A01513-1 anti-CD31/Pecam1 antibody is tested on mouse ovary tissue, stomach tissue, kidney tissue. It is guaranteed to work for ELISA, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-11

Question

Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-CD31/Pecam1 antibody A01513-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-24

Question

Would A01513-1 anti-CD31/Pecam1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Anderson

Verified customer

Asked: 2019-08-21

Answer

As indicated on the product datasheet, A01513-1 anti-CD31/Pecam1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-21

Question

We have observed staining in mouse tendon of biceps brachii. What should we do? Is anti-CD31/Pecam1 antibody supposed to stain tendon of biceps brachii positively?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

According to literature tendon of biceps brachii does express PECAM1. According to Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2019-07-22

Question

I was wanting to use your anti-CD31/Pecam1 antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?

G. Anderson

Verified customer

Asked: 2018-11-16

Answer

You can see on the product datasheet, A01513-1 anti-CD31/Pecam1 antibody has been validated for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-16

Question

Would anti-CD31/Pecam1 antibody A01513-1 work for WB with leukemic t-cell?

B. Thomas

Verified customer

Asked: 2018-10-19

Answer

According to the expression profile of leukemic t-cell, PECAM1 is highly expressed in leukemic t-cell. So, it is likely that anti-CD31/Pecam1 antibody A01513-1 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2018-10-19

Question

Is this A01513-1 anti-CD31/Pecam1 antibody reactive to the isotypes of PECAM1?

P. Rodriguez

Verified customer

Asked: 2018-03-22

Answer

The immunogen of A01513-1 anti-CD31/Pecam1 antibody is E. coli-derived mouse CD31 recombinant protein (Position: R58-Q273). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-03-22

Question

We are interested in to test anti-CD31/Pecam1 antibody A01513-1 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-CD31/Pecam1 antibody A01513-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-24

Answer

The products we sell, including anti-CD31/Pecam1 antibody A01513-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-24

Question

Is a blocking peptide available for product anti-CD31/Pecam1 antibody (A01513-1)?

K. Zhao

Verified customer

Asked: 2017-11-16

Answer

We do provide the blocking peptide for product anti-CD31/Pecam1 antibody (A01513-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-16

Question

We bought anti-CD31/Pecam1 antibody for WB on tendon of biceps brachii a few years ago. I am using mouse, and We want to use the antibody for ELISA next. We want examining tendon of biceps brachii as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

I have checked the website and datasheets of our anti-CD31/Pecam1 antibody and I see that A01513-1 has been tested on mouse in both WB and ELISA. Thus A01513-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-06-14

Question

My colleagues were happy with the WB result of your anti-CD31/Pecam1 antibody. However we have observed positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is PECAM1 supposed to be expressed?

J. Miller

Verified customer

Asked: 2013-06-17

Answer

Based on literature, lung does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2013-06-17

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A01513-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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