Click image to see more details
Product Info Summary
Customers Who Bought This Also Bought
Anti-CD31/Pecam1 Antibody Picoband™
Boster Bio Anti-CD31/Pecam1 Antibody Picoband™ catalog # A01513-1. Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD31/Pecam1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01513-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived mouse CD31 recombinant protein (Position: R58-Q273).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01513-1 is reactive to Pecam1 in Mouse
A01513-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (A01513-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse ovary tissue lysates,
Lane 2: mouse stomach tissue lysates,
Lane 3: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # A01513-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 130KD. The expected band size for CD31 is at 82KD.
Gene/Protein Information For Pecam1 (Source: Uniprot.Org, NCBI)
Platelet endothelial cell adhesion molecule
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule Pecam1|C85791, Cd31, PECAM-1, Pecam|platelet/endothelial cell adhesion molecule 1|platelet endothelial cell adhesion molecule*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Pecam1, check out the Pecam1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Pecam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A01513-1 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shen Song,Zhao Liu,Mohamed Aamer Abubaker,Ling Ding,Ji Zhang,Shengrong Yang,Zengjie Fan,Antibacterial polyvinyl alcohol/bacterial cellulose/ nano-silver hydrogels that effectively promote wound healing,Materials Science and Engineering: C,Volume 126,2021,112171,ISSN 0928-4931,https://doi.org/10.1016/j.msec.2021.112171.
A01513-1 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:NA
Wang L,Wu H,Xiong L,Liu X,Yang N,Luo L,Qin T,Zhu X,Shen Z,Jing H,Chen J.Quercetin Downregulates Cyclooxygenase-2 Expression and HIF-1α/VEGF Signaling-Related Angiogenesis in a Mouse Model of Abdominal Aortic Aneurysm.Biomed Res Int.2020 Aug 22;2020:9485398.doi:10.1155/2020/9485398.PMID:32908926;PMCID:PMC7463408.
A01513-1 usage in article: APP:IHC, SAMPLE:ABDOMINAL AORTA TISSUE, DILUTION:1:100
MicroRNA-210 promotes angiogenesis in acute myocardial infarction
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase
The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex
Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling
ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer
Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing
WSS25 Inhibits Growth of Xenografted Hepatocellular Cancer Cells in Nude Mice by Disrupting Angiogenesis via Blocking Bone Morphogenetic Protein (BMP)/Smad/Id1 Signaling
Chen X, Zhang M, Chen S, Wang X, Tian Z, Chen Y, Xu P, Zhang L, Zhang L, Zhang L. Cell Transplant. 2017 Aug;26(8):1331-1340. doi: 10.1177/0963689717721216. Peptide-Modified Chitosan Hydrogels Accelerate Skin Wound Healing by Promoting Fibroblast P...
Chen L, Chan Th, Yuan Yf, Hu L, Huang J, Ma S, Wang J, Dong Ss, Tang Kh, Xie D, Li Y, Guan Xy. J Clin Invest. 2010 Apr;120(4):1178-91. Doi: 10.1172/Jci40665. Epub 2010 Mar 24. Chd1L Promotes Hepatocellular Carcinoma Progression And Metastasis In M...
Qiu H, Yang B, Pei Zc, Zhang Z, Ding K. J Biol Chem. 2010 Oct 15;285(42):32638-46. Doi: 10.1074/Jbc.M110.105544. Epub 2010 Aug 2. Wss25 Inhibits Growth Of Xenografted Hepatocellular Cancer Cells In Nude Mice By Disrupting Angiogenesis Via Blocking...
Zhou S, Yi T, Liu R, Bian C, Qi X, He X, Wang K, Li J, Zhao X, Huang C, Wei Y. Mol Cell Proteomics. 2012 Jul;11(7):M112.017988. Doi: 10.1074/Mcp.M112.017988. Epub 2012 Apr 9. Proteomics Identification Of Annexin A2 As A Key Mediator In The Metasta...
Xiu J, Fan J, Li J, Cui G, Lei W. Biomed Res Int. 2014;2014:785146. Doi: 10.1155/2014/785146. Epub 2014 Jun 10. Different Angiogenic Abilities Of Self-Setting Calcium Phosphate Cement Scaffolds Consisting Of Different Proportions Of Fibrin Glue.
Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.
Wu Q, Chang Y, Zhang L, Zhang Y, Tian T, Feng G, Zhou S, Zheng Q, Han F, Huang F. J Cancer. 2013 Nov 21;4(9):727-35. Doi: 10.7150/Jca.7576. Ecollection 2013. Srpk1 Dissimilarly Impacts On The Growth, Metastasis, Chemosensitivity And Angiogenesis O...
Chen J, Jia Zy, Ma Zl, Wang Yy, Teng Gj. Plos One. 2011;6(6):E20790. Doi: 10.1371/Journal.Pone.0020790. Epub 2011 Jun 24. In Vivo Serial Mr Imaging Of Magnetically Labeled Endothelial Progenitor Cells Homing To The Endothelium Injured Artery In Mice.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-CD31/Pecam1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-CD31/Pecam1 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question