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- Table of Contents
Pecam1 antibody (A01513-2) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IF analysis of CD31 using anti-CD31 antibody (A01513-2).
Paraffin-embedded mouse skin sections were stained for CD31 in three experimental groups: (1) normal control, (2) congestion group, in which the skin was treated with ethanol, and (3) inflammation group, stimulated with LPS. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD31 Antibody (A01513-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody incubated with 1:500 for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Paraffin-embedded mouse skin sections were stained for CD31 in three experimental groups: (1) normal control, (2) congestion group, in which the skin was treated with ethanol, and (3) inflammation group, stimulated with LPS. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD31 Antibody (A01513-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody incubated with 1:500 for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CD31/Pecam1 Antibody Picoband®
Pecam1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Pecam1 antibody was positively detected by WB in:
rat lung tissue, rat spleen tissue, rat heart tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Pecam1 antibody was positively detected by IHC in:
mouse colon tissue, mouse kidney tissue, rat colon tissue, rat heart tissue, rat kidney tissue, rat liver tissue, rat lung tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-CD31/Pecam1 Antibody Picoband®
View all PECAM1 Antibodies
View all PECAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD31/Pecam1 recombinant protein (Position: Q41-E258).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100-120 kDa
Calculated Molecular Weight
81.3 kDa
Background of Pecam1
CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily and this gene is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.
Cite This Product
Anti-CD31/Pecam1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01513-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
78%
Moderate Homology
Dog
63%
Low homology
Monkey
63%
Low homology
Human
61%
Low homology
Rabbit
61%
Low homology
Cow
60%
Low homology
Sheep
60%
Low homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Pecam1 antibody
Immunohistochemistry (IHC) protocol for Pecam1 antibody
Publications
Application:
Species:
High-sensitivity spin-exchange relaxation-free (SERF) magnetometry combined with magnetic-responsive iron oxide nanoparticles for real-time monitoring of malignant tumor therapy
Published: 2026-01-01
Journal: Theranostics
Application: IF
Impact Factor: 13.30
Species: Mouse
PMID: 41355967
EPAC inhibitor suppresses angiogenesis and tumor growth of triple-negative breast cancer
Published: 2024-03-04
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: IHC,IF
Impact Factor: 4.20
Species: Mouse
PMID: 38447883
The impact of bone marrow-derived mesenchymal stem cells on neovascularisation in rats with brain injury
Published: 2018-06-28
Journal: FOLIA NEUROPATHOLOGICA
Application: FC
Impact Factor: 1.35
Species: Rat
PMID: 30509031
Review
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2 Reviews For Anti-CD31/Pecam1 Antibody Picoband®
The Anti-CD31 antibody (Cat# A01513-2) shows high specificity in mouse lung IF, clearly labeling endothelial cells with accurate localization.
| SKU | A01513-2 |
|---|---|
| Application | Immunofluorescence |
| Sample | mouse lung tissue |
| Sample Processing Description | Left lung from normal mouse was washed 3 times with PBS to remove blood cells, fixed in formaldehyde for 24 h, and paraffin-embedded for sectioning. |
| Other Reagents | Goat serum, DAPI (Cat# AR1176, Boster Bio) |
| Primary Antibody | CD31/Pecam1 Antibody Picoband® |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (Cat# BA1142, Boster Bio) |
| Secondary Incubation | 1:500, 45min in 37℃ |
| Detection | Image system:Leica DM2500 |
| Results Summary | CD31, also known as PECAM-1, is a key marker of vascular endothelial cells and plays a central role in biomedical research, especially in angiogenesis and tumor microenvironment studies. In this experiment, CD31 IF was performed to label endothelial cells, facilitating subsequent evaluation of vascular density distribution. The results show clear endothelial staining with precise localization, yielding excellent outcomes. |
Fei Liu, The First Affiliated Hospital of Nanchang University
Verified customer
Submitted 2026-03-24
IF using Anti-CD31 antibody (A01513-2) showed specific, strong, and stable endothelial staining, revealing expanded and inflamed microvessels in treated mouse skin compared to controls.
| SKU | A01513-2 |
|---|---|
| Application | Immunofluorescence |
| Sample | Paraffin-embedded mouse skin sections |
| Sample Processing Description | Mouse skin samples were divided into three groups: (i) normal control, (ii) congestion group, treated with ethanol, and (iii) inflammation group, stimulated with LPS. |
| Other Reagents | Goat serum, DAPI, Anti-fade mounting medium |
| Primary Antibody | CD31/Pecam1 Antibody Picoband® |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | DyLight 488–conjugated Goat Anti-Rabbit IgG (H+L)) (Boster, BA1127) |
| Secondary Incubation | 1:500, 45 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | IF staining with Boster CD31 antibody produced high-quality vascular images. In the control group, vessels were small and well-defined. In the congestion group (ethanol-treated), CD31 staining revealed markedly dilated vascular lumens. In the inflammation group (LPS-treated), vessel dilation was accompanied by changes in tissue spacing. Combined with Prussian blue staining and magnetic signal measurements, we observed that despite extreme vessel dilation in the congestion group, magnetic signals were not significantly elevated; only the inflammation group, with extensive macrophage infiltration and uptake, showed high magnetic signals. |
Wenbo Wang, Shandong University
Verified customer
Submitted 2026-01-30
Product Promise
We promise Anti-CD31/Pecam1 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01513-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026