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- Table of Contents
TRPV5 antibody (A03218-1) performs well in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HepG2 cells using anti-TRPV5 antibody (A03218-1).
Overlay histogram showing HepG2 cells stained with A03218-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV5 Antibody (A03218-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with A03218-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV5 Antibody (A03218-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TRPV5 Antibody Picoband®
TRPV5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB |
Tested Applications
TRPV5 antibody was positively detected by WB in:
monkey COS-7 whole cell, human U-87MG whole cell, human Hela whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TRPV5 antibody was positively detected by IHC in:
human pancreatic cancer tissue, mouse kidney tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TRPV5 antibody was positively detected by FC in:
HepG2 cell, U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
ELISA
Monkey
Tested
Human
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-TRPV5 Antibody Picoband®
View all TRPV5 Antibodies
View all TRPV5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPV5 recombinant protein (Position: Q14-Q74+H636-F729).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated Molecular Weight
82.6 kDa
Background of TRPV5
Transient receptor potential cation channel subfamily V member 5is aproteinthat in humans is encoded by the TRPV5 gene. This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. And this protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. In addition, TRPV5 is mainly expressed inkidneyepithelial cells, where it plays an important role in the reabsorption of Ca2+. Genetic deletion of TRPV5 in mice leads to Ca2+loss in the urine, and consequentialhyperparathyroidism, and bone loss.
Cite This Product
Anti-TRPV5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03218-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Rabbit
80%
Moderate Homology
Dog
79%
Moderate Homology
Rat
74%
Moderate Homology
Mouse
73%
Moderate Homology
Cow
71%
Moderate Homology
Sheep
71%
Moderate Homology
Zebrafish
22%
Low homology
Drosophila
16%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TRPV5 antibody
Immunohistochemistry (IHC) protocol for TRPV5 antibody
Flow Cytometry (FC) protocol for TRPV5 antibody
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Product Promise
We promise Anti-TRPV5 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03218-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026