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Product Info Summary
| SKU: | A08149 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Ataxin-2L ATXN2L Antibody
SKU/Catalog Number
A08149
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ataxin-2L ATXN2L Antibody catalog # A08149. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ataxin-2L ATXN2L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08149)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ATXN2L. AA range:571-620
Reactive Species
A08149 is reactive to ATXN2L in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
113.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08149 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of 293T cells using Ataxin-2L Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from NIH/3T3 and RAW264.7 cells, using ATXN2L Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using ATXN2L antibody.
Specific Publications For Anti-Ataxin-2L ATXN2L Antibody (A08149)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Ataxin-2L ATXN2L Antibody
Question
Would anti-Ataxin-2L antibody A08149 work on primate WB with lymph?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Our lab technicians have not validated anti-Ataxin-2L antibody A08149 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Ataxin-2L antibody A08149 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lymph in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-Ataxin-2L antibody A08149 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The anti-Ataxin-2L antibody (A08149) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-18
Question
I was wanting to use your anti-Ataxin-2L antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
You can see on the product datasheet, A08149 anti-Ataxin-2L antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-24
Question
I see that the anti-Ataxin-2L antibody A08149 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-04-11
Question
Would anti-Ataxin-2L antibody A08149 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
According to the expression profile of cervix carcinoma, ATXN2L is highly expressed in cervix carcinoma. So, it is likely that anti-Ataxin-2L antibody A08149 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-12-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Ataxin-2L antibody A08149. Let me know if you need anything else.
R. Gonzalez
Verified customer
Asked: 2017-11-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-24
