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- Table of Contents
TRPV1 antibody (A00128-4) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of U87 cells using anti-TRPV1 antibody (A00128-4).
Overlay histogram showing U87 cells stained with A00128-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV1 Antibody (A00128-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with A00128-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV1 Antibody (A00128-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TRPV1 Antibody Picoband®
TRPV1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
TRPV1 antibody was positively detected by WB in:
human 293T whole cell, human HepG2 whole cell, rat liver tissue, rat PC-12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TRPV1 antibody was positively detected by ICC/IF in:
T-47D cell
TRPV1 antibody was positively detected by FC in:
U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Sheep
Predicted
Predicted
Bovine
Predicted
Predicted
Mouse
Predicted
Predicted
Product Information
Product Name
Anti-TRPV1 Antibody Picoband®
View all TRPV1 Antibodies
View all TRPV1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPV1 recombinant protein (Position: E107-A800).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
95.0 kDa
Background of TRPV1
The transient receptor potential cation channel, subfamily V, member 1(TRPV1), also known as the capsaicin receptor is a protein which in humans is encoded by the TRPV1 gene. TRPV1(also called Vanilloid receptor type 1) is a ligand-gated nonselective cation channel that is considered to be an important integrator of various pain stimuli such as endogenous lipids, capsaicin, heat, and low pH. In addition to expression in primary afferents, TRPV1 is also expressed in the CNS. Cui M et al.(2006) demonstrate that TRPV1 receptors in the CNS plays an important role in pain mediated by central sensitization. And the significant CNS penetration is necessary for a TRPV1 antagonist to produce broad-spectrum analgesia. And TRPV1 also participates in normal bladder function and is essential for normal mechanically evoked purinergic signaling by the urothelium.
Cite This Product
Anti-TRPV1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00128-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
94%
High Homology
Rabbit
93%
High Homology
Sheep
92%
High Homology
Cow
92%
High Homology
Mouse
92%
High Homology
Rat
91%
High Homology
Zebrafish
52%
Low homology
Drosophila
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TRPV1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TRPV1 antibody
Flow Cytometry (FC) protocol for TRPV1 antibody
Publications
Application:
Species:
TRPV1 mediates oxidative stress and autophagy pathways to regulate airway MUC5AC secretion
Published: 2025-12-17
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB,IF
Impact Factor: 5.60
Species: Human,Mouse
PMID: 41418942
Targeting TRPV1 channels in desensitized neural afferent pathways may help mitigate pain and lower urinary tract symptoms caused by prostatitis
Published: 2025-02-25
Journal: Frontiers in Pharmacology
Application: IF
Impact Factor: 4.80
Species: Rat
PMID: 40070569
A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson’s disease
Published: 2025-01-15
Journal: Science Advances
Application: IF
Impact Factor: 12.50
Species: Rat
PMID: 39813330
Preparation of dual supramolecular EGCG-carboxymethyl chitosan: Structure, antioxidant and anti-inflammatory properties
Published: 2025-01-06
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IF
Impact Factor: 8.50
Species: Human
PMID: 39778836
Interaction of high temperature and NO2 exposure on asthma risk: In vivo experimental evidence of inflammation and oxidative stress
Published: 2023-01-23
Journal: SCIENCE OF THE TOTAL ENVIRONMENT
Application: IHC
Impact Factor: 9.80
Species: Mouse
PMID: 36702287
Ion channel-targeting near-infrared photothermal switch with synergistic effect for specific cancer therapy
Published: 2022-01-06
Journal: Journal of Materials Chemistry B
Impact Factor: 7.57
Species: Human
PMID: 35022632
Review
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1 Review For Anti-TRPV1 Antibody Picoband®
The Anti-TRPV1 antibody (A00128-4) showed clear and correctly positioned bands in WB of MADB106 rat breast cancer cells, with increased TRPV1 expression upon agonist treatment and decreased expression upon inhibitor treatment.
| SKU | A00128-4 |
|---|---|
| Application | Western blot |
| Sample | Rat MADB106 breast cancer cells |
| Sample Processing Description | Normally cultured MADB106 cells, MADB106 cells treated with an agonist, MADB106 cells treated with an inhibitor |
| Other Reagents | RIPA lysis buffer (Cat. No. AR0102, Bosterbio, Wuhan) Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | TRPV1 Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG |
| Secondary Incubation | 1h at 37℃ |
| Detection | Substrate: ECL substrate, Imaging system:ChemiDoc MP |
| Results Summary | The role of TRPV1 in breast cancer research is highly complex, varying with cell type, tumor subtype, and tumor microenvironment, and in some cases even showing contradictory effects. The current consensus is that TRPV1 is not only a potential therapeutic target for breast cancer but also an important prognostic and regulatory factor. Experimental results show that TRPV1 protein expression markedly increases in cells treated with an agonist, while it decreases in cells treated with an inhibitor. |
Xinshuo Wang, Xi’an Jiaotong University
Verified customer
Submitted 2026-02-12
Product Promise
We promise Anti-TRPV1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00128-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026