Anti-beta-Actin ACTB Antibody

Product Name

Anti-beta-Actin ACTB Antibody

View all ACTB Antibodies

SKU/Catalog Number

A01263-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-beta-Actin ACTB Antibody (Catalog # A01263-2). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-beta-Actin ACTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-2)

Host

Rabbit

Contents

beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin.

Reactive Species

A01263-2 is reactive to ACTB in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

41.7 kDa

Background of ACTB

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01263-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ACTB

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