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Product Info Summary
| SKU: | A00565 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TRPV4 Antibody
SKU/Catalog Number
A00565
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRPV4 Antibody (Catalog # A00565). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRPV4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TRPV4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00565)
Host
Rabbit
Contents
TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4. The immunogen is located within amino acids 380 - 430 of TRPV4.
Cross-reactivity
TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.
Reactive Species
A00565 is reactive to TRPV4 in Human, Mouse, Rat
Observed Molecular Weight
98 kDa
Calculated molecular weight
98.3 kDa
Background of TRPV4
The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00565 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC-P: 1-2 μg/mL; IHC-P/IF: 5-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in the human sample. All other applications and species not yet tested.
Validation Images & Assay Conditions
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WB Validation in Human Cells Loading: 15 μg of lysate Antibodies: TRPV4, A00565, 0.5 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Human Tissues Loading: 15 μg of lysate Antibodies: TRPV4, A00565, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Mouse Tissues Loading: 15 μg of lysate Antibodies: TRPV4, A00565, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Rat Tissues Loading: 15 μg of lysate Antibodies: TRPV4, A00565, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of TRPV4 in Human Liver Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TRPV4 antibody (A00565) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of TRPV4 in Mouse Kidney Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRPV4 antibody (A00565) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of TRPV4 in Rat Kidney Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TRPV4 antibody (A00565) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 μg/ml.
Red: TRPV4 Antibody (A00565)
Blue: DAPI staining
Specific Publications For Anti-TRPV4 Antibody (A00565)
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