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Product Info Summary
| SKU: | A13747 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Tryptase-3 Antibody
SKU/Catalog Number
A13747
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Tryptase-3 Antibody catalog # A13747. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tryptase-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13747)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Tryptase-3 at AA range: 51-100
Reactive Species
A13747 is reactive to TPSD1 in Human
Calculated molecular weight
26.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13747 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Western Blot analysis of HEPG2 cells using Tryptase-3 Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200
Specific Publications For Anti-Tryptase-3 Antibody (A13747)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Tryptase-3 Antibody
Question
I was wanting to use your anti-Tryptase-3 antibody for IHC for human esophagogastric junction muscularis propria on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagogastric junction muscularis propria identification?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
You can see on the product datasheet, A13747 anti-Tryptase-3 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagogastric junction muscularis propria in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-04
Question
Is this A13747 anti-Tryptase-3 antibody reactive to the isotypes of TPSD1?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The immunogen of A13747 anti-Tryptase-3 antibody is Synthesized peptide derived from Tryptase-3 at AA range: 51-100. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-17
Question
My question regarding product A13747, anti-Tryptase-3 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13747 anti-Tryptase-3 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-21
Question
We are currently using anti-Tryptase-3 antibody A13747 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
The anti-Tryptase-3 antibody (A13747) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-23
