Human ADAMTS1 ELISA Kit PicoKine®

ADAMTS1 ELISA kit for Human

Human ADAMTS1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAMTS1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 2 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK1340
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human ADAMTS1 ELISA Kit PicoKine®

View all ADAMTS1 ELISA kits

SKU/Catalog Number

EK1340

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ADAMTS1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAMTS1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ADAMTS1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1340)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: F253-A734

Sensitivity

<10 pg/ml

Assay Range

93.8 pg/ml - 6,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1340 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1340 is reactive to ADAMTS1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1340 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ADAMTS1

ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

Kit Components

Catalog Number Description Quantity
EK1340-CAP Anti-Human ADAMTS1 Pre-coated 96-well strip microplate 1
EK1340-ST Human ADAMTS1 Standard 2 vials, 10 ng/tube
EK1340-DA Human ADAMTS1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.7187.5375750150030006000
O.D.0.0480.0960.1390.2240.4460.6671.2071.993

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect ADAMTS1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 93.8 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ADAMTS1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14568128861327322843
Standard deviation8.8437.45207.7910.5651.97213.22
CV (%)6.1%5.5%7.2%8%7.1%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ADAMTS1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11451441551491484.322.9%
Sample 268161066366365426.584%
Sample 328862884269026072766121.834.4%
*number of samples for each test n=16.

Gene/Protein Information For ADAMTS1 (Source: Uniprot.Org, NCBI)

Gene Name

ADAMTS1

Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 1

Weight

105.358kDa

Alternative Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1 ADAMTS1 C3-C5, METH1 ADAM metallopeptidase with thrombospondin type 1 motif 1 A disintegrin and metalloproteinase with thrombospondin motifs 1|ADAM-TS 1|ADAM-TS1|ADAMTS-1|METH-1|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1|human metalloproteinase with thrombospondin type 1 motifs

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADAMTS1, check out the ADAMTS1 Infographic

ADAMTS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADAMTS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1340 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pro-atherogenic proteoglycanase ADAMTS-1 is down-regulated by lauric acid through PI3K and JNK signaling pathways in THP-1 derived macrophages

Tang, H., Lee, M., Kim, E.H., Bishop, D., & Rodgers, G.M. (2017). siRNA-knockdown of ADAMTS-13 modulates endothelial cell angiogenesis. Microvascular Research, 113, 65-70. Advance online publication. doi: 10.1016/j.mvr.2017.05.007

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1 Reviews For Human ADAMTS1 ELISA Kit PicoKine®

0

Human ADAMTS1 PicoKine ™ ELISA Kit Worked Well--Huiyuan Tang, University of Utah, Hematology and Hematologic Malignancies, Lab Specialist

Excellent

Source: Biocompare.com

Applications ELISA
Sample Cell lysates from HUVEC treated with control siRNA or ADAMTS-13 siRNA
Detection --

"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")

Verified customer

Submitted

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8 Customer Q&As for Human ADAMTS1 ELISA Kit PicoKine®

Question

Is this immunogen sequence (F253-A734) used to produce both the monoclonal capture antibody and the polyclonal detection antibody? and has the eptitope of the monoclonal antibody been mapped?

Verified customer

Asked: 2020-09-24

Answer

The immunogen sequence of monoclonal capture antibody and polyclonal detection antibody is F253-C724 Accession # Q9UHI8. The eptitope of the monoclonal antibody hasn't been mapped. The standard protein used in the kit formed from the immunogen sequence (F253-A734).

Boster Scientific Support

Answered: 2020-09-25

Question

Q: What is the optimal O.D. value for ADAMTS1 ELISA kit? I used your ADAMTS1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain ADAMTS1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ADAMTS1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ADAMTS1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-08-12

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement ADAMTS1 using the EK1340 Human ADAMTS1 Picokine® ELISA Kit?

M. Chen

Verified customer

Asked: 2019-06-08

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-06-08

Question

Q: how to proceed with the analysis of ELISA data? I have performed ADAMTS1 level in plasma.

Verified Customer

Verified customer

Asked: 2019-05-04

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-05-04

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Wang

Verified customer

Asked: 2018-08-18

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-08-18

Question

Q: which procedure should I follow in order to thaw whole blood sample for ADAMTS1 ELISA after freezing?

B. Roberts

Verified customer

Asked: 2017-05-09

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ADAMTS1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-05-09

Question

Q: if the enzyme conjugated ADAMTS1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ADAMTS1 antigen?

W. Bhasin

Verified customer

Asked: 2017-04-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-04-14

Question

Q: is it okay to use EDTA plasma as samples in Human ADAMTS1 Picokine® ELISA Kit (Catalog # EK1340)?

P. Gandhi

Verified customer

Asked: 2016-04-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of ADAMTS1 causing disruption of its protein structure. ADAMTS1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ADAMTS1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-04-09

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