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Product Info Summary
| SKU: | EK1340 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human ADAMTS1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1340
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ADAMTS1 ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAMTS1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ADAMTS1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1340)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F253-A734
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
93.8 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1340 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1340 is reactive to ADAMTS1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1340 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ADAMTS1
ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1340-CAP | Anti-Human ADAMTS1 Pre-coated 96-well strip microplate | 1 |
| EK1340-ST | Human ADAMTS1 Standard | 2 vials, 10 ng/tube |
| EK1340-DA | Human ADAMTS1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.048 | 0.107 | 0.151 | 0.229 | 0.42 | 0.729 | 1.366 | 2.138 |
Data Example Images
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Human ADAMTS1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ADAMTS1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 93.8 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ADAMTS1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 145 | 681 | 2886 | 132 | 732 | 2843 |
| Standard deviation | 8.84 | 37.45 | 207.79 | 10.56 | 51.97 | 213.22 |
| CV (%) | 6.1% | 5.5% | 7.2% | 8% | 7.1% | 7.5% |
Specific Publications For Human ADAMTS1 ELISA Kit PicoKine® (EK1340)
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Customer Reviews
1 Reviews For Human ADAMTS1 ELISA Kit PicoKine®
Human ADAMTS1 PicoKine ™ ELISA Kit Worked Well--Huiyuan Tang, University of Utah, Hematology and Hematologic Malignancies, Lab Specialist
Excellent
Source: Biocompare.com
| SKU | EK1340 |
|---|---|
| Applications | ELISA |
| Sample | Cell lysates from HUVEC treated with control siRNA or ADAMTS-13 siRNA |
| Detection | -- |
"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")
Huiyuan Tang
Verified customer
Submitted 2017-08-21
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Human ADAMTS1 ELISA Kit PicoKine®
Question
Is this immunogen sequence (F253-A734) used to produce both the monoclonal capture antibody and the polyclonal detection antibody? and has the eptitope of the monoclonal antibody been mapped?
Verified customer
Asked: 2020-09-24
Answer
The immunogen sequence of monoclonal capture antibody and polyclonal detection antibody is F253-C724 Accession # Q9UHI8. The eptitope of the monoclonal antibody hasn't been mapped. The standard protein used in the kit formed from the immunogen sequence (F253-A734).
Boster Scientific Support
Answered: 2020-09-25
Question
Q: What is the optimal O.D. value for ADAMTS1 ELISA kit? I used your ADAMTS1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain ADAMTS1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ADAMTS1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ADAMTS1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-08-12
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement ADAMTS1 using the EK1340 Human ADAMTS1 Picokine® ELISA Kit?
M. Chen
Verified customer
Asked: 2019-06-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-06-08
Question
Q: how to proceed with the analysis of ELISA data? I have performed ADAMTS1 level in plasma.
Verified Customer
Verified customer
Asked: 2019-05-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2019-05-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Wang
Verified customer
Asked: 2018-08-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-18
Question
Q: which procedure should I follow in order to thaw whole blood sample for ADAMTS1 ELISA after freezing?
B. Roberts
Verified customer
Asked: 2017-05-09
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ADAMTS1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-05-09
Question
Q: if the enzyme conjugated ADAMTS1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ADAMTS1 antigen?
W. Bhasin
Verified customer
Asked: 2017-04-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-04-14
Question
Q: is it okay to use EDTA plasma as samples in Human ADAMTS1 Picokine® ELISA Kit (Catalog # EK1340)?
P. Gandhi
Verified customer
Asked: 2016-04-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of ADAMTS1 causing disruption of its protein structure. ADAMTS1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ADAMTS1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-04-09
