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- Table of Contents
TPH1 antibody (A01626-1) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.
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Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®
TPH1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
TPH1 antibody was positively detected by WB in:
human SGC-7901 whole cell, rat heart tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®
View all TPH1 Antibodies
View all TPH1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Tryptophan Hydroxylase recombinant protein (Position: M1-K111).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated Molecular Weight
51.0 kDa
Background of TPH1
Tryptophan hydroxylase 1 (TPH1) is an isoenzyme of tryptophan hydroxylase which in humans is encoded by the TPH1 gene. This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.
Cite This Product
Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01626-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
95%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
91%
High Homology
Rat
88%
Moderate Homology
Mouse
84%
Moderate Homology
Zebrafish
69%
Low homology
Drosophila
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TPH1 antibody
Publications
Application:
Species:
Ligilactobacillus murinus alleviates binge-eating behavior by restoring serotonin metabolism
Published: 2026-02-12
Journal: FOOD RESEARCH INTERNATIONAL
Application: WB
Impact Factor: 8.00
Species: Mouse,Rat
PMID: 41819877
Review
Have you used Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®?
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1 Review For Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®
WB results showed that TPH1 (A01626-1) was upregulated in the rat colon model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.
| SKU | A01626-1 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE. |
| Other Reagents | 5% non-fat milk |
| Primary Antibody | Tryptophan Hydroxylase/TPH1 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:5000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system:ChemiDoc MP |
| Results Summary | The figure shows WB results of TPH1 and the internal control Actin in rat colon across different groups; expression was elevated in the model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results. |
Shiyu Zhang, Liaoning University of Traditional Chinese Medicine
Verified customer
Submitted 2026-03-04
Product Promise
We promise Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01626-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026