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Product Info Summary
| SKU: | M04167 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB, FACS |
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Product info
Product Name
Anti-TSLP Receptor Antibody (Monoclonal, ABM28B6)
SKU/Catalog Number
M04167
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-TSLP Receptor Antibody (Monoclonal, ABM28B6) (Catalog# M04167). Tested in FACS, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-TSLP Receptor Antibody (Monoclonal, ABM28B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04167)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABM28B6
Isotype
IgG2a Kappa
Immunogen
A partial length recombinant TSLPR protein (amino acids 97-252)
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M04167 is reactive to CRLF2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.0 kDa
Background of CRLF2
CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04167 is guaranteed for WB, FACS Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Flow cytometry: 0.5-1 µg/10^6 Cells
Validation Images & Assay Conditions
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Western blot analysis of TSLP Receptor. Anti-TSLP Receptor antibody (Clone: ABM28B6) was tested at 2 &mu
Specific Publications For Anti-TSLP Receptor Antibody (Monoclonal, ABM28B6) (M04167)
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4 Customer Q&As for Anti-TSLP Receptor Antibody (Monoclonal, ABM28B6)
Question
Will anti-TSLP Receptor antibody (Monoclonal, ABM28B6) M04167 work for WB with t-cell?
S. Krishna
Verified customer
Asked: 2016-07-05
Answer
According to the expression profile of t-cell, CRLF2 is highly expressed in t-cell. So, it is likely that anti-TSLP Receptor antibody (Monoclonal, ABM28B6) M04167 will work for WB with t-cell.
Boster Scientific Support
Answered: 2016-07-05
Question
Is this M04167 anti-TSLP Receptor antibody (Monoclonal, ABM28B6) reactive to the isotypes of CRLF2?
J. Mangal
Verified customer
Asked: 2016-02-08
Answer
The immunogen of M04167 anti-TSLP Receptor antibody (Monoclonal, ABM28B6) is A partial length recombinant TSLPR protein (amino acids 97-252). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-02-08
Question
Would anti-TSLP Receptor antibody (Monoclonal, ABM28B6) M04167 work on primate WB with lung?
C. Krishna
Verified customer
Asked: 2015-07-29
Answer
Our lab technicians have not tested anti-TSLP Receptor antibody (Monoclonal, ABM28B6) M04167 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TSLP Receptor antibody (Monoclonal, ABM28B6) M04167 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-29
Question
Will M04167 anti-TSLP Receptor antibody (Monoclonal, ABM28B6) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Kulkarni
Verified customer
Asked: 2014-09-15
Answer
It shows on the product datasheet, M04167 anti-TSLP Receptor antibody (Monoclonal, ABM28B6) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-09-15
