Anti-UBE2J1/Ubc6E Antibody

UBE2J1 antibody

Boster Bio Anti-UBE2J1/Ubc6E Antibody catalog # A07059. Tested in IP, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A07059
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: IP, WB

Product Name

Anti-UBE2J1/Ubc6E Antibody

View all UBE2J1 Antibodies

SKU/Catalog Number

A07059

Size

100ug

Form

Liquid

Description

Boster Bio Anti-UBE2J1/Ubc6E Antibody catalog # A07059. Tested in IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UBE2J1/Ubc6E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07059)

Host

Rabbit

Contents

0.02 M Potassium Phosphate
0.15 M Sodium Chloride
pH 7.2
0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07059 is reactive to UBE2J1 in Human

Applications

A07059 is guaranteed for IP, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blot, 1:200 - 1:2, 000, human
Direct ELISA, 1:1, 000 - 1:5, 000, human
ImmunoPrecipitation: 1-2μg, human

Validation Images & Assay Conditions

Gene/Protein Information For UBE2J1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9Y385

Gene ID

51465

Gene Name

UBE2J1

Full Name

Ubiquitin-conjugating enzyme E2 J1

Weight

35.199kDa

Superfamily

ubiquitin-conjugating enzyme family

Alternative Names

CGI-76; EC 6.3.2.19; HSPC153; HSPC205; HSU93243; HsUBC6e; MGC12555; NCUBE1; NCUBE-1; NCUBE1HSUBC6e; Non-canonical ubiquitin-conjugating enzyme 1; non-canonical ubquitin conjugating enzyme 1; UBC6e; Ubc6p; UBE2J1; ubiquitin-conjugating enzyme E2 J1; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast); Yeast ubiquitin-conjugating enzyme UBC6 homolog E UBE2J1 CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, UBC6E, Ubc6p ubiquitin conjugating enzyme E2 J1 ubiquitin-conjugating enzyme E2 J1|E2 ubiquitin-conjugating enzyme J1|HSUBC6e|non-canonical ubiquitin-conjugating enzyme 1|ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)|ubiquitin-conjugating enzyme E2, J1, U|yeast ubiquitin-conjugating enzyme UBC6 homolog E

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on UBE2J1, check out the UBE2J1 Infographic

UBE2J1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UBE2J1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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4 Customer Q&As for Anti-UBE2J1/Ubc6E Antibody

Question

I was wanting to use your anti-UBE2J1/Ubc6E antibody for IP for human spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen identification?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

It shows on the product datasheet, A07059 anti-UBE2J1/Ubc6E antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-05

Question

I see that the anti-UBE2J1/Ubc6E antibody A07059 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-07

Question

Would A07059 anti-UBE2J1/Ubc6E antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

It shows on the product datasheet, A07059 anti-UBE2J1/Ubc6E antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-04

Question

We are currently using anti-UBE2J1/Ubc6E antibody A07059 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

The anti-UBE2J1/Ubc6E antibody (A07059) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-20

Size

Total: $479

SKU:A07059

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A07059
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$479.00

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