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Product Info Summary
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Boster Bio Anti-UBE2J1/Ubc6E Antibody catalog # A07059. Tested in IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2J1/Ubc6E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07059)
0.02 M Potassium Phosphate
0.15 M Sodium Chloride
0.01% (w/v) Sodium Azide
A synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A07059 is reactive to UBE2J1 in Human
A07059 is guaranteed for IP, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blot, 1:200 - 1:2, 000, human
Direct ELISA, 1:1, 000 - 1:5, 000, human
ImmunoPrecipitation: 1-2μg, human
Validation Images & Assay Conditions
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Western blot analysis of UBE2J1 expression in lysate from mock-transfected 293 cells (lane 1) and 293 cells over-expressing Myc-Ube2j1 (Lane 2). UBE2J1 at 35-40KD was detected using rabbit anti-UBE2J1 Antigen Affinity purified polyclonal antibody (Catalog # A07059). Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.
Gene/Protein Information For UBE2J1 (Source: Uniprot.org, NCBI)
Ubiquitin-conjugating enzyme E2 J1
ubiquitin-conjugating enzyme family
CGI-76; EC 220.127.116.11; HSPC153; HSPC205; HSU93243; HsUBC6e; MGC12555; NCUBE1; NCUBE-1; NCUBE1HSUBC6e; Non-canonical ubiquitin-conjugating enzyme 1; non-canonical ubquitin conjugating enzyme 1; UBC6e; Ubc6p; UBE2J1; ubiquitin-conjugating enzyme E2 J1; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast); Yeast ubiquitin-conjugating enzyme UBC6 homolog E UBE2J1 CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, UBC6E, Ubc6p ubiquitin conjugating enzyme E2 J1 ubiquitin-conjugating enzyme E2 J1|E2 ubiquitin-conjugating enzyme J1|HSUBC6e|non-canonical ubiquitin-conjugating enzyme 1|ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)|ubiquitin-conjugating enzyme E2, J1, U|yeast ubiquitin-conjugating enzyme UBC6 homolog E*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on UBE2J1, check out the UBE2J1 Infographic
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In this infographic, you will see the following information for UBE2J1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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