Rabbit IgG polyclonal antibody for Polyubiquitin-B(UBB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Anti-Ubiquitin/UBB Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Ubiquitin/UBB Antibody
See all UBB primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Ubiquitin/UBB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Ubiquitin/UBB information: Molecular Weight: 25762 MW; Subcellular Localization: Ubiquitin: Cytoplasm . Nucleus .|
|Cite This Product||Anti-Ubiquitin/UBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1420)|
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|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Ubiquitin(29-45aa KIQDKEGIPPDQQRLIF), identical to the rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For UBB (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7314|
|Species Of This Entry||Human|
|Alternative Names||UBB|RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin|
|Post Tranlational Modifications||Cleavage; Methylation|
|Subcellular Localization||[Ubiquitin]: Cytoplasm. Nucleus.|
|Gene Location||17p11.2, on Chromasome 17, gene sequence: NC_000017.11 (16380793..16382745)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For UBB (Source: Uniprot.org, NCBI)
|Protein Function||Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.|
|Interacting Proteins||CCDC50; DAZAP2; DUSP1; GGA1; GGA3; Mast2; MCM7; Pax3; RABGEF1; RAD23B; RNF11; RNF43; SMURF2; UBAC1|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Anemia; Liver Carcinoma; Anemia, Diamond-blackfan; Aplasia, Nos; Adenoma; Epidermolysis Bullosa; Junctional Epidermolysis Bullosa; Disorders Of Sex Development; Hepatitis B; Carcinogenesis; Dysplasia; Colorectal Cancer; Epidermolysis Bullosa Dystrophica; Porphyria, Erythropoietic; Congenital Absence|
|Related Pathways||Translation; Cell Cycle; Cell Adhesion; Gene Silencing; Proteolysis; Larval Development; Oocyte Maturation; Methylation; Cell Death; Cell Growth; Mitotic Cell Cycle; Rna Processing; Rna Interference|
|Background||Ubiquitin(originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues(ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.|
|Scientific References||PMID: 3029682 by Baker R.T., et al. The human ubiquitin gene family: structure of a gene and pseudogenes from the Ub B subfamily.|
PMID: 14745543 by Tachikui H., et al. Lineage-specific homogenization of the polyubiquitin gene among human and great apes.
Our Boster Quality Guarantee for Anti-Ubiquitin/UBB Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Human MCF-7 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
IHC(P): Human Lung Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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