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Product Info Summary
| SKU: | M01432-3 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328
SKU/Catalog Number
M01432-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328 (Catalog # M01432-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01432-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM328
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to C-terminus of human Cytokeratin-14
Cross-reactivity
This antibody reacts to human Cytokeratin-14. It may also react to mouse or rat Cytokeratin-14, as predicted by immunogen homology.
Reactive Species
M01432-3 is reactive to KRT14 in Human
Calculated molecular weight
51.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01432-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of A431 cell lysate using anti-CK-14 rabbit monoclonal antibody (Clone RM328) at a 1:1000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-14 rabbit monoclonal antibody (Clone RM328) at a 1:2000 dilution.
Specific Publications For Anti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328 (M01432-3)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328
Question
Is there a BSA free version of anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 available?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
D. Miller
Verified customer
Asked: 2020-04-15
Answer
The anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 (M01432-3) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-15
Question
I see that the anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-02
Question
Is this M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 reactive to the isotypes of KRT14?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The immunogen of M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 is A peptide corresponding to C-terminus of human Cytokeratin-14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-31
Question
Is a blocking peptide available for product anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 (M01432-3)?
J. Moore
Verified customer
Asked: 2019-12-11
Answer
We do provide the blocking peptide for product anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 (M01432-3). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-11
Question
See attached the WB image, lot number and protocol we used for pancreas skin using anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas skin using anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
Does anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 work for WB with pancreas skin?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of pancreas skin, KRT14 is highly expressed in pancreas skin. So, it is likely that anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 will work for WB with pancreas skin.
Boster Scientific Support
Answered: 2019-07-19
Question
My lab would like to test anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 on rat pancreas skin for research purposes, then I may be interested in using anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 M01432-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
I was wanting to use your anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 for WB for rat pancreas skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat pancreas skin identification?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can see on the product datasheet, M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-12
Question
We have been able to see staining in human lymphoblast. Any tips? Is anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 supposed to stain lymphoblast positively?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Based on literature lymphoblast does express KRT14. Based on Uniprot.org, KRT14 is expressed in gingival epithelium, brain, pancreas skin, epidermis, lymphoblast, cervix carcinoma, liver, among other tissues. Regarding which tissues have KRT14 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Epidermis, Pubmed ID: 6186381
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2019-05-24
Question
I have a question about product M01432-3, anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-15
Question
My boss were content with the WB result of your anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
From literature, pancreas skin does express KRT14. Generally KRT14 expresses in cytoplasm. nucleus. note=expressed in both. Regarding which tissues have KRT14 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Epidermis, Pubmed ID: 6186381
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2017-08-29
Question
We bought anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
I looked at the website and datasheets of our anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2017-06-07
Question
We are interested in using your anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 for hemidesmosome assembly studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
L. Zhang
Verified customer
Asked: 2017-03-27
Answer
We appreciate your inquiry. This M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 is tested on a431 cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-03-27
Question
Would M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Lewis
Verified customer
Asked: 2016-07-25
Answer
It shows on the product datasheet, M01432-3 anti-CK-14 (Cytokeratin-14) Rabbit Monoclonal antibody, Clone#RM328 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-25
