Anti-USP25 Antibody

Product Name

Anti-USP25 Antibody

View all USP25 Antibodies

SKU/Catalog Number

A06182

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-USP25 Antibody (Catalog # A06182). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-USP25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06182)

Host

Rabbit

Contents

USP25 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25.

Cross-reactivity

USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

Reactive Species

A06182 is reactive to USP25 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

122.2 kDa

Background of USP25

USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06182 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information USP25

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