USP25 Antibodies

AND USP25 ELISA kits, USP25 Proteins

Many biological assays use antibodies to detect USP25, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with USP25 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop USP25 antibodies...

We validate the specificity of these antibodies to USP25 by testing them on tissues known to express USP25 positively and negatively. Browse below to find the USP25 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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USP25 Infographic by Boster Bio

All USP25 Products

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Specificity

Applications

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Anti-USP25 Antibody Picoband®

Western blot analysis of USP25 using anti-USP25 antibody (A06182-1)

IF analysis of USP25 using anti-USP25 antibody (A06182-1)

Flow Cytometry analysis of HL-60 cells using anti-USP25 antibody (A06182-1)

Human, Monkey, Mouse, Rat

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A06182-1
100 μg/vial

Anti-USP25 Antibody

Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide

Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 μg/mL

Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 μg/mL

Human, Mouse

ELISA, IF, IHC-P, WB

$481
Cat # A06182
0.1 mg

Anti-USP25 Antibody

Western blot analysis of extracts of various cells, using USP25 antibody

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug USP25 antibody

Immunohistochemistry of paraffin-embedded mouse brain using USP25 antibody at dilution of 1:100 (x40 lens)

Human, Mouse, Rat

IHC, WB

$405
Cat # A06182-2
100ul

USP25 (NM_013396) Human Recombinant Protein

Coomassie blue staining of purified USP25 protein

$1249
Cat # PROTQ9UHP3
20 µg

Anti-USP25 Antibody

Western blot analysis of lysate from Daudi cell line, using USP25 Antibody

Human

$299
Cat # M06182
80 µl

USP25 (NM_013396) Human Over-expression Lysate

Human

$628
Cat # LS029761
20 µg, 100 µg

USP25 Overview

Facts about Ubiquitin carboxyl-terminal hydrolase 25.

USP25 3D structure. Source: alphafold

Ubiquitin carboxyl-terminal hydrolase 25 (USP25)

Deubiquitinating enzyme which hydrolyzes ubiquitin moieties conjugated to substrates and so, works to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both'Lys-48'- and'Lys-63'-connected tetraubiquitin chains.

Gene Information

Human
Gene Name:	USP25
Uniprot:	Q9UHP3
Entrez:	29761

Family

Belongs to:

peptidase C19 family

Alternative Names

Deubiquitinating enzyme 25; EC 3.1.2.15; EC 3.4.19.12; ubiquitin carboxyl-terminal hydrolase 25; ubiquitin specific peptidase 25; ubiquitin specific protease USP2510USP21ubiquitin specific protease 25; ubiquitin thioesterase 25; Ubiquitin Thiolesterase 25; ubiquitin-specific processing protease 25; Ubiquitin-specific-processing protease 25; USP on chromosome 21; USP25

Molecular Weight

Mass (kDA):
122.218 kDA

Genomic Context


Human

Location:	21q21.1
Sequence:	21; NC_000021.9 (15729934..15880072)

Tissue Specificity

Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.

Subcellular Localizations

Cytoplasm.; [Isoform USP25m]: Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.

Diseases

Down Syndrome
Malignant Neoplasms
Autoimmunity
Liver Diseases
Malignant Neoplasm Of Breast
Herpes Labialis
Aneuploidy
Malignant Neoplasm Of Lung
Carcinoma
Endoplasmic Reticulum Stress
Myopathy
Nonalcoholic Fatty Liver Disease
Fibrosis

Neoplasms
Infectious Canine Hepatitis
Mammary Neoplasms
Bulla
Fatty Liver
Anxiety Disorders
Lung Neoplasms

Interacting Proteins

MAGEB4
RAD23A
RAD23B
SUMO2
SYK

ZNF426

Pathways

Pathogenesis
Conjugation
Notch Signaling Pathway
Cell Death
Proteolysis
Protein Ubiquitination

PTMs

Ubiquitination

Phosphorylation

Sumoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/USP25

References

PMID: 10644437 by Valero R., et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2.

PMID: 10612803 by Groet J., et al. Narrowing of the region of allelic loss in 21q11-21 in squamous non- small cell lung carcinoma and cloning of a novel ubiquitin-specific protease gene from the deleted segment.