Click image to see more details
Product Info Summary
| SKU: | M01372 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8]
SKU/Catalog Number
M01372
Size
100 µl
Description
Boster Bio VAPB mouse monoclonal antibody, clone OTI4D8 (formerly 4D8). Catalog# M01372. Tested in WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01372)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4D8
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 2-223 of human VAPB (NP_004729) produced in E.coli.
Reactive Species
M01372 is reactive to VAPB in Human, Mouse, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01372 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of VAPB using anti-VAPB antibody (M01372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-VAPB antigen affinity purified monoclonal antibody (Catalog # M01372) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for VAPB at approximately 27 kDa. The expected band size for VAPB is at 27 kDa.
Specific Publications For Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8] (M01372)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8]?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-VAPB Mouse Monoclonal Antibody [Clone ID: OTI4D8]
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
