Anti-Alpha Defensin 1/DEFA1 Antibody Picoband®

Product Name

Anti-Alpha Defensin 1/DEFA1 Antibody Picoband®

View all DEFA1 Antibodies

SKU/Catalog Number

A10546

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha Defensin 1/DEFA1 Antibody Picoband® catalog # A10546. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

DEFA1 (alpha defensin 1; HNP-1) is described as part of a defensin family of antimicrobial/cytotoxic peptides involved in host defense, abundant in neutrophil granules and also found at mucosal epithelia; strong sequence similarity within the defensin family and copy number variation for clustered alpha defensin genes. Assay context: validated for WB (observed ~19 kDa; calculated ~10.2 kDa). In inflammatory profiling, DEFA1 is commonly interpreted alongside cytokine markers such as IL1B or IL1A; for quantitative sandwich formats, matched antibody pairs are typically required (general principle; see antibody pairs development for methodological context).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha Defensin 1/DEFA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10546)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Alpha Defensin 1.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10546 is reactive to DEFA1 in Human, Rat

Observed Molecular Weight

19 kDa

Calculated molecular weight

10.2 kDa

Background of DEFA1

Defensin, alpha 1, also known as human alpha defensin 1, human neutrophil peptide 1 (HNP-1) or neutrophil defensin 1 is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10546 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: rat testis tissue, HELA whole cell

Validation Images & Assay Conditions

See full target information DEFA1

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