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Product Info Summary
| SKU: | M00303 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-VASP Rabbit Monoclonal Antibody
SKU/Catalog Number
M00303
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VASP Rabbit Monoclonal Antibody catalog # M00303. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VASP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00303)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BHO-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VASP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00303 is reactive to VASP in Human
Applications
M00303 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
62 kDa
Calculated molecular weight
39.83kDa
Background of VASP
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of VASP expression in HepG2 cell lysate.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For VASP (Source: Uniprot.org, NCBI)
Gene Name
VASP
Full Name
Vasodilator-stimulated phosphoprotein
Weight
39.83kDa
Superfamily
Ena/VASP family
Alternative Names
vasodilator-stimulated phosphoprotein; VASP VASP vasodilator stimulated phosphoprotein vasodilator-stimulated phosphoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VASP, check out the VASP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VASP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VASP Rabbit Monoclonal Antibody (M00303)
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No publications found for M00303
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-VASP Rabbit Monoclonal Antibody
Question
We have observed staining in human leukemic t-cell. Any tips? Is anti-VASP Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
From what I have seen in literature leukemic t-cell does express VASP. From what I have seen in Uniprot.org, VASP is expressed in blood, promyelocyte, substantia nigra, fetal lung, fetal spleen skin, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VASP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal lung, Fetal spleen, and Skin, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801, 18088087
Promyelocyte, Pubmed ID: 7828592
Substantia nigra, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-03
Question
Does M00303 anti-VASP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
It shows on the product datasheet, M00303 anti-VASP Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
Is this M00303 anti-VASP Rabbit Monoclonal antibody reactive to the isotypes of VASP?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The immunogen of M00303 anti-VASP Rabbit Monoclonal antibody is A synthesized peptide derived from human VASP . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-09
Question
See attached the WB image, lot number and protocol we used for liver using anti-VASP Rabbit Monoclonal antibody M00303. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Does anti-VASP Rabbit Monoclonal antibody M00303 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
According to the expression profile of liver, VASP is highly expressed in liver. So, it is likely that anti-VASP Rabbit Monoclonal antibody M00303 will work for WB with liver.
Boster Scientific Support
Answered: 2019-11-06
Question
Our lab were well pleased with the WB result of your anti-VASP Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma cytoplasm. cytoplasm using this antibody. Is that expected? Could you tell me where is VASP supposed to be expressed?
Z. Williams
Verified customer
Asked: 2019-08-07
Answer
From what I have seen in literature, cervix carcinoma does express VASP. Generally VASP expresses in cytoplasm. cytoplasm, cytoskeleton. cell. Regarding which tissues have VASP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal lung, Fetal spleen, and Skin, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801, 18088087
Promyelocyte, Pubmed ID: 7828592
Substantia nigra, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-07
Question
We bought anti-VASP Rabbit Monoclonal antibody for WB on liver a few months ago. I am using human, and We intend to use the antibody for IP next. I am interested in examining liver as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
I took a look at the website and datasheets of our anti-VASP Rabbit Monoclonal antibody and I see that M00303 has been validated on human in both WB and IP. Thus M00303 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-04
Question
I see that the anti-VASP Rabbit Monoclonal antibody M00303 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-02
Question
Is a blocking peptide available for product anti-VASP Rabbit Monoclonal antibody (M00303)?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
We do provide the blocking peptide for product anti-VASP Rabbit Monoclonal antibody (M00303). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-15
Question
I was wanting to use your anti-VASP Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
As indicated on the product datasheet, M00303 anti-VASP Rabbit Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-13
Question
Do you have a BSA free version of anti-VASP Rabbit Monoclonal antibody M00303 available?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VASP Rabbit Monoclonal antibody M00303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-30
Question
Does anti-VASP Rabbit Monoclonal antibody M00303 work on zebrafish IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
Our lab technicians have not validated anti-VASP Rabbit Monoclonal antibody M00303 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-VASP Rabbit Monoclonal antibody M00303 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish leukemic t-cell in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-29
Question
We need to test anti-VASP Rabbit Monoclonal antibody M00303 on human liver for research purposes, then I may be interested in using anti-VASP Rabbit Monoclonal antibody M00303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
The products we sell, including anti-VASP Rabbit Monoclonal antibody M00303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-VASP Rabbit Monoclonal antibody M00303. Let me know if you need anything else.
A. Li
Verified customer
Asked: 2015-10-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-13
Question
We want using your anti-VASP Rabbit Monoclonal antibody for cell junction assembly studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
D. Zhang
Verified customer
Asked: 2015-01-13
Answer
We appreciate your inquiry. This M00303 anti-VASP Rabbit Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for IP, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-13
Question
My question regarding product M00303, anti-VASP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Collins
Verified customer
Asked: 2014-07-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00303 anti-VASP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-16
Question
We are currently using anti-VASP Rabbit Monoclonal antibody M00303 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
J. Collins
Verified customer
Asked: 2013-02-14
Answer
The anti-VASP Rabbit Monoclonal antibody (M00303) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-14
