Mouse LOX-1/OLR1 ELISA Kit PicoKine™

Mouse LOX-1/OLR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Olr1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Product Info Summary

SKU: EK0825
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse LOX-1/OLR1 ELISA Kit PicoKine™

See all LOX-1/OLR1 products

SKU/Catalog Number

EK0825

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse LOX-1/OLR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Olr1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse LOX-1/OLR1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0825)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R60-I363

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0825 is reactive to Olr1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0825 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0825-CAP Anti-Mouse Olr1 Pre-coated 96-well strip microplate 1
EK0825-ST Mouse Olr1 Standard 2 vials, 10 ng/tube
EK0825-DA Mouse Olr1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0360.0640.1200.2050.3620.6811.0101.819

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse LOX-1/OLR1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8532176785312750
Standard deviation4.8417.3360.595.3522.4661.5
CV (%)8.2%5.4%7.9%7.2%7.2%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of LOX-1/OLR1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 19510295104994.064.1%
Sample 232130428429630113.444.4%
Sample 376779481978879218.532.3%
*number of samples for each test n=16.

Gene/Protein Information For Olr1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9EQ09

Gene ID

108078

Gene Name

Olr1

Full Name

Oxidized low-density lipoprotein receptor 1

Weight

41.643kDa

Alternative Names

CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1 Olr1|LOX, LOX-1, SR, SR-EI, Sca, Scare1|oxidized low density lipoprotein (lectin-like) receptor 1|oxidized low-density lipoprotein receptor 1|lectin-like oxLDL receptor 1|lectin-like oxidized LDL receptor 1|lectin-type oxidized LDL receptor 1|ox-LDL receptor 1|oxidised low density lipoprotein (lectin-like) receptor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Olr1, check out the Olr1 Infographic

Olr1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Olr1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse LOX-1/OLR1 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for LOX-1 ELISA kit? I performed your LOX-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain LOX-1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-06-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this LOX-1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of LOX-1 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2020-06-10

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis LOX-1 using the EK0825 Mouse LOX-1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-02-22

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-02-22

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

M. Chen

Verified customer

Asked: 2019-09-12

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-09-12

Question

Q: Can LOX-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-07-24

Question

Q: if the enzyme conjugated LOX-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LOX-1 antigen?

J. Thompson

Verified customer

Asked: 2019-05-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-05-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-11-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-11-26

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

T. Kulkarni

Verified customer

Asked: 2018-06-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-21

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