Click image to see more details
Product Info Summary
| SKU: | A00691-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Proto-oncogene vav VAV1 Antibody
SKU/Catalog Number
A00691-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Proto-oncogene vav VAV1 Antibody catalog # A00691-1. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Proto-oncogene vav VAV1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00691-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
5B5
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human VAV1. AA range:131-180
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00691-1 is reactive to VAV1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
98.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00691-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of extracts from NIH-3T3 cells, using VAV1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Immunofluorescence analysis of HeLa cells, using VAV1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Proto-oncogene vav VAV1 Antibody (A00691-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Proto-oncogene vav VAV1 Antibody
Question
Is a blocking peptide available for product anti-VAV1 antibody (A00691-1)?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We do provide the blocking peptide for product anti-VAV1 antibody (A00691-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-20
Question
I have a question about product A00691-1, anti-VAV1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00691-1 anti-VAV1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-08
Question
Does A00691-1 anti-VAV1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can see on the product datasheet, A00691-1 anti-VAV1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-16
Question
We want to test anti-VAV1 antibody A00691-1 on mouse spleen for research purposes, then I may be interested in using anti-VAV1 antibody A00691-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Lewis
Verified customer
Asked: 2019-06-11
Answer
The products we sell, including anti-VAV1 antibody A00691-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-11
Question
I see that the anti-VAV1 antibody A00691-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-16
