Anti-Neutrophil Elastase/ELANE Antibody Picoband™

Boster Bio Anti-Neutrophil Elastase/ELANE Antibody Picoband™ catalog # PB10058. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: PB10058
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Neutrophil Elastase/ELANE Antibody Picoband™

See all Neutrophil Elastase/ELA2 products

SKU/Catalog Number

PB10058

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neutrophil Elastase/ELANE Antibody Picoband™ catalog # PB10058. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neutrophil Elastase/ELANE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10058)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB10058 is reactive to Elane in Mouse, Rat

Applications

PB10058 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Elane (Source: Uniprot.Org, NCBI)

Uniprot ID

Q3UP87

Gene ID

50701

Gene Name

Elane

Full Name

Neutrophil elastase

Weight

28.648kDa

Superfamily

peptidase S1 family

Alternative Names

Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1 Elane|El, Ela2, F430011M15Rik, N, NE|elastase, neutrophil expressed|neutrophil elastase|elastase 2, neutrophil|leukocyte elastase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Elane, check out the Elane Infographic

Elane infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Elane: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB10058

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6 Customer Q&As for Anti-Neutrophil Elastase/ELANE Antibody Picoband™

Question

I have a question about product PB10058, anti-Neutrophil Elastase/ELANE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Jackson

Verified customer

Asked: 2018-06-26

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10058 anti-Neutrophil Elastase/ELANE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-26

Question

Would anti-Neutrophil Elastase/ELANE antibody PB10058 work on canine WB with lung?

Verified Customer

Verified customer

Asked: 2018-03-28

Answer

Our lab technicians have not validated anti-Neutrophil Elastase/ELANE antibody PB10058 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Neutrophil Elastase/ELANE antibody PB10058 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine lung in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-28

Question

We are currently using anti-Neutrophil Elastase/ELANE antibody PB10058 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

The anti-Neutrophil Elastase/ELANE antibody (PB10058) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-26

Question

Would PB10058 anti-Neutrophil Elastase/ELANE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-07

Answer

You can see on the product datasheet, PB10058 anti-Neutrophil Elastase/ELANE antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow cell using anti-Neutrophil Elastase/ELANE antibody PB10058. Let me know if you need anything else.

D. Anderson

Verified customer

Asked: 2014-03-12

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-03-12

Question

I see that the anti-Neutrophil Elastase/ELANE antibody PB10058 works with WB, what is the protocol used to produce the result images on the product page?

M. Singh

Verified customer

Asked: 2013-01-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-01-08

Conjugation

how to order through PO

Size

Total: $280

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

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PB10058
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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