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Cdh5 antibody (A02632-2) performs well in IHC, WB, ELISA (Cap) and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 24 Publications

a02632 2 cdh5 primary antibodies ihc testing 2

IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2).
VE Cadherin was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VE Cadherin Antibody (A02632-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-VE-Cadherin Cdh5-Antibody Picoband®

Cdh5 antibody

24 Publications

Reactive Species	Host	Application
Mouse, Rat	Rabbit	IHC, WB, ELISA (Cap)

Tested Applications

Cdh5 antibody was positively detected by WB in:

mouse lung tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Cdh5 antibody was positively detected by IHC in:

mouse lung tissue, rat lung tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Mouse

Tested

Recommend

Rat

Tested

Recommend

Product Information

Product Name

Anti-VE-Cadherin Cdh5-Antibody Picoband®
View all CDH5 Antibodies

Form

Lyophilized

Uniprot

P55284

Gene ID

12562

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse VE Cadherin recombinant protein (Position: D46-E285).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

120 kDa

Calculated Molecular Weight

87.9 kDa

Background of Cdh5

CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.

Cite This Product

Anti-VE-Cadherin Cdh5-Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02632-2)

Protocols

Western Blot protocol for Cdh5 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Cdh5 antibody

Review Protocol

Publications

Correction: Detrimental effect of sitagliptin induced autophagy on multiterritory perforator flap survival

Published: 2026-01-23

Journal: Frontiers in Pharmacology

Application: WB,IHC

Impact Factor: 4.80

Species: Rat

PMID: 41657769

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Free Secondary Antibody

Anti-VE-Cadherin Cdh5-Antibody Picoband®

Cdh5 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Correction: Detrimental effect of sitagliptin induced autophagy on multiterritory perforator flap survival

Cascade-Enhanced Pt Nanozyme Platform Anchored on Microgels for Effective Lactate Depletion and EndoMT Attenuation Post-Myocardial Infarction

miR-142 regulates the function of microvascular endothelial cells through MAT2B

Cordycepin promotes the survival of random-pattern skin flaps via autophagy in a rat model

Microenvironment-adaptive nanomedicine MXene promotes flap survival by inhibiting ROS cascade and endothelial pyroptosis

Pharmacodynamic insights into maresin 1: Enhancing flap viability via the keap1/Nrf2 axis to control ROS-driven apoptosis and ferroptosis

Celastrol enhances the viability of random-pattern skin flaps by regulating autophagy through the AMPK-mTOR-TFEB axis

Dihydrocapsaicin suppresses the STING-mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia–reperfusion injury of perforator skin flap

Rosuvastatin promotes survival of random skin flaps through AMPK-mTOR pathway-induced autophagy

Effects of limonin treatment on the survival of random skin flaps in mice

Protein kinase D1 promotes the survival of random-pattern skin flaps in rats

Therapeutic potential of pravastatin for random skin flaps necrosis: involvement of promoting angiogenesis and inhibiting apoptosis and oxidative stress

Catalpol Enhances Random-Pattern Skin Flap Survival by Activating SIRT1-Mediated Enhancement of Autophagy

Pseudoginsenoside F11 Enhances the Viability of Random-Pattern Skin Flaps by Promoting TFEB Nuclear Translocation Through AMPK-mTOR Signal Pathway

Targeting TFE3 Protects Against Lysosomal Malfunction-Induced Pyroptosis in Random Skin Flaps via ROS Elimination

Butylphthalide Inhibits Autophagy and Promotes Multiterritory Perforator Flap Survival

Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment

Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study

Detrimental Effect of Sitagliptin Induced Autophagy on Multiterritory Perforator Flap Survival

FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival

miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1

Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway

Salvianolic Acid B Promotes the Survival of Random-Pattern Skin Flaps in Rats by Inducing Autophagy

Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling

Review

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