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Product Info Summary
| SKU: | PA1827 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Myoglobin/MB Antibody Picoband®
SKU/Catalog Number
PA1827
BA3791 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myoglobin/MB Antibody catalog # PA1827. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myoglobin/MB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1827)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Myoglobin, identical to the related rat sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1827 is reactive to Mb in Human, Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
17.1 kDa
Background of Mb
Myoglobin (MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It is a member of the globin superfamily and is expressed in skeletal and cardiac muscles. This gene is mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1827 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Positive Control
WB: human placenta tissue, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue
IHC: mouse heart tissue, rat heart tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of Myoglobin/MB using anti-Myoglobin/MB antibody (PA1827).
Myoglobin/MB was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin/MB Antibody (PA1827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Western blot analysis of Myoglobin using anti-Myoglobin antibody (PA1827).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myoglobin antigen affinity purified polyclonal antibody (PA1827) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Myoglobin at approximately 17 kDa. The expected band size for Myoglobin is at 17 kDa.
Click image to see more details
IHC analysis of Myoglobin using anti-Myoglobin antibody (PA1827).
Myoglobin was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin Antibody (PA1827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Myoglobin using anti-Myoglobin antibody (PA1827).
Myoglobin was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin Antibody (PA1827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Myoglobin/MB Antibody Picoband® (PA1827)
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Customer Q&As
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15 Customer Q&As for Anti-Myoglobin/MB Antibody Picoband®
Question
We bought anti-Myoglobin/MB antibody for WB on heart right ventricle last year. I am using rat, and I plan to use the antibody for IHC next. We want examining heart right ventricle as well as heart in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
A. Taylor
Verified customer
Asked: 2020-04-15
Answer
I have checked the website and datasheets of our anti-Myoglobin/MB antibody and it appears that PA1827 has been validated on rat in both WB and IHC. Thus PA1827 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Myoglobin/MB antibody PA1827. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
We are interested in using your anti-Myoglobin/MB antibody for response to hypoxia studies. Has this antibody been tested with western blotting on rat heart tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We appreciate your inquiry. This PA1827 anti-Myoglobin/MB antibody is validated on rat heart tissue, skeletal muscle tissue, tissue lysate, liver tissue, intestine tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-19
Question
I have a question about product PA1827, anti-Myoglobin/MB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1827 anti-Myoglobin/MB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
We have seen staining in mouse skeletal muscle. Are there any suggestions? Is anti-Myoglobin/MB antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
According to literature skeletal muscle does express MB. According to Uniprot.org, MB is expressed in heart right ventricle, skeletal muscle, heart, among other tissues. Regarding which tissues have MB expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 7895732
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-05
Question
Is this PA1827 anti-Myoglobin/MB antibody reactive to the isotypes of MB?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The immunogen of PA1827 anti-Myoglobin/MB antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Myoglobin(138-154aa LFRNDIAAKYKELGFQG), identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-18
Question
Will anti-Myoglobin/MB antibody PA1827 work for WB with heart?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
According to the expression profile of heart, MB is highly expressed in heart. So, it is likely that anti-Myoglobin/MB antibody PA1827 will work for WB with heart.
Boster Scientific Support
Answered: 2019-11-14
Question
Is a blocking peptide available for product anti-Myoglobin/MB antibody (PA1827)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do provide the blocking peptide for product anti-Myoglobin/MB antibody (PA1827). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-24
Question
I was wanting to use your anti-Myoglobin/MB antibody for WB for rat heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat heart identification?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can see on the product datasheet, PA1827 anti-Myoglobin/MB antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-28
Question
Would PA1827 anti-Myoglobin/MB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Brown
Verified customer
Asked: 2018-10-01
Answer
As indicated on the product datasheet, PA1827 anti-Myoglobin/MB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-01
Question
I see that the anti-Myoglobin/MB antibody PA1827 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-14
Question
Do you have a BSA free version of anti-Myoglobin/MB antibody PA1827 available?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Myoglobin/MB antibody PA1827 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-26
Question
See below the WB image, lot number and protocol we used for heart using anti-Myoglobin/MB antibody PA1827. Please let me know if you require anything else.
L. Johnson
Verified customer
Asked: 2017-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-28
Question
I am looking for to test anti-Myoglobin/MB antibody PA1827 on rat heart for research purposes, then I may be interested in using anti-Myoglobin/MB antibody PA1827 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
The products we sell, including anti-Myoglobin/MB antibody PA1827, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-01
Question
We are currently using anti-Myoglobin/MB antibody PA1827 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on feline tissues as well?
R. Mangal
Verified customer
Asked: 2014-04-14
Answer
The anti-Myoglobin/MB antibody (PA1827) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-14
