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Product Info Summary
| SKU: | M01734 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4)
SKU/Catalog Number
M01734
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) catalog # M01734. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01734)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6C4
Isotype
Mouse IgG1
Immunogen
E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01734 is reactive to Fabp3 in Mouse, Rat
Observed Molecular Weight
15 kDa
Calculated molecular weight
14.8 kDa
Background of Fabp3
Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01734 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: mouse heart tissue, mouse heart tissue, rat heart tissue, rat heart tissue
IHC: rat cardiac muscle tissue
Validation Images & Assay Conditions
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Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cardiac FABP antigen affinity purified monoclonal antibody (Catalog # M01734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
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IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734).
Cardiac FABP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cardiac FABP Antibody (M01734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Sandwich ELISA - Recombinant rat Cardiac FABP protein standard curve.
Use in combination with reagents from Rat Cardiac FABP ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1623).
Specific Publications For Anti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) (M01734)
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