Skip to the beginning of the images gallery

VIM antibody (M00235-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 42 Publications

Datasheet MSDS Request COA

m00235 1 vimentin primary antibodies if testing 18

View All Images (28)

Immunofluorescent analysis of Hela cells, using Vimentin Antibody.

Designing a Western blot for VIM? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the VIM Western Blot Guide

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Vimentin Rabbit Monoclonal Antibody

VIM antibody

42 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

VIM antibody was positively detected by WB in:

human 293T whole cell, human U251 whole cell, human U-87MG whole cell, human Jurkat whole cell, rat lung tissue, mouse lung tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

VIM antibody was positively detected by IHC in:

human colon cancer tissue, human glioma tissue, human liver cancer tissue, human non small cell lung cancer tissue, human ovarian cancer tissue, human placenta tissue, human tonsil tissue, rat brain tissue, rat lung tissue, human liver cancer tissue, human lung cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, human cervical cancer tissue, human pancreatic cancer tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

VIM antibody was positively detected by ICC/IF in:

Hela cell

VIM antibody was positively detected by IF in:

human tonsil tissue, human placenta tissue, mouse brain tissue, human liver caner tissue, human lung caner tissue, human thyroid caner tissue, human cervical caner tissue, human pancreatic caner tissue, human stomach caner tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Tested

Recommend

Product Information

Product Name

Anti-Vimentin Rabbit Monoclonal Antibody
View all VIM Antibodies

Form

Liquid

Uniprot

P08670

Gene ID

7431

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

AGF-22

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Vimentin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

54 kDa

Calculated Molecular Weight

53.7 kDa

Cite This Product

Anti-Vimentin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-1)

Protocols

Western Blot protocol for VIM antibody

Review Protocol

Immunohistochemistry (IHC) protocol for VIM antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for VIM antibody

Review Protocol

Immunofluorescence (IF) protocol for VIM antibody

Review Protocol

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Published: 2026-08-26

Journal: CLINICAL AND EXPERIMENTAL MEDICINE

Application: WB

Impact Factor: 4.50

Species: Human,Mouse

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Vimentin Rabbit Monoclonal Antibody

VIM antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Role of Ca2+‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/EDNRB

Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer

Cytological Effects of Cadmium Poisoning and the Protective Effect of Quercetin: A Mechanism Exploration based on the Testicular Lamina Propria

LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts

Construction of Lentiviral Vectors Carrying Six Pluripotency Genes in Yak to Obtain Yak iPSC Cells

Form-Deprivation Myopia Promotes Sclera M2-type Macrophages Polarization in Mice

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

Inhibition of Notch3/Hey1 ameliorates peribiliary hypoxia by preventing hypertrophic hepatic arteriopathy in biliary atresia progression

CircFOXO3 mediates hypoxia-induced autophagy of endometrial stromal cells in endometriosis

Juan-tong-yin potentially impacts endometriosis pathophysiology by enhancing autophagy of endometrial stromal cells via unfolded protein reaction-triggered endoplasmic reticulum stress

miRNA-130a-3p/CPEB4 Axis Modulates Glioblastoma Growth and Progression

The Effect of Resveratrol in Sirt1/CST Pathway to Inhibit TNF-α Induced Inflammatory Response in Rat Primary Fibroblast-Like Synoviocytes

Shenlian extract improves atherosclerosis by relieving adventitial inflammation

Efficient intervention for pulmonary fibrosis via mitochondrial transfer promoted by mitochondrial biogenesis

Artesunate protects against ocular fibrosis by suppressing fibroblast activation and inducing mitochondria-dependent ferroptosis

Effect of TGF- β 2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia

Activation of AMP-activated protein kinase ablated the formation of aortic dissection by suppressing vascular inflammation and phenotypic switching of vascular smooth muscle cells

Targeted inhibition of ZAK ameliorates renal interstitial fibrosis

Negative pressure wound therapy enhances bone regeneration compared with conventional therapy in a rabbit radius gap‑healing model

MicroRNA-223 negatively regulates LPS-induced inflammatory responses by targeting NLRP3 in human dental pulp fibroblasts

Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge

HER4 promotes the progression of colorectal cancer by promoting epithelial‑mesenchymal transition

Nanocrystallized Oleanolic Acid Better Inhibits Proliferation, Migration and Invasion in Intracranial Glioma via Caspase-3 Pathway

Dexamethasone Provides Effective Immunosuppression for Improved Survival of Retinal Organoids after Epiretinal Transplantation

Melatonin enhances hydrogen peroxide-induced apoptosis in human dental pulp cells

Melatonin regulates mitochondrial function and biogenesis during rat dental papilla cell differentiation.

Botulinum toxin type A prevents the phenotypic transformation of fibroblasts induced by TGF‑β1 via the PTEN/PI3K/Akt signaling pathway

A Comparative Evaluation of Concentrated Growth Factor and Platelet-rich Fibrin on the Proliferation, Migration, and Differentiation of Human Stem Cells of the Apical Papilla

High-temperature Requirement Protein A1 Regulates Odontoblastic Differentiation of Dental Pulp Cells via the Transforming Growth Factor Beta 1/Smad Signaling Pathway

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Effect of interleukin-1β and lipoxin A4 in human endometriotic stromal cells: Proteomic analysis

Substance P Inhibits the Collagen Synthesis of Rat Myocardial Fibroblasts Induced by Ang II

Expression of E-cadherin and vimentin in oral squamous cell carcinoma

Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

Role of protein kinase C β2 in relaxin-mediated inhibition of cardiac fibrosis

Down-regulation of Aquaporin1 (AQP1) by peptidoglycan via p38 MAPK pathways in primary rat pleural mesothelial cells

Effect of GnRH-II on the ESC proliferation, apoptosis and VEGF secretion in patients with endometriosis in vitro

MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1β induced in human endometriosis stromal cells in vitro

Disclosure of a stem cell phenotype in an oral squamous cell carcinoma cell line induced by BMP-4 via an epithelial-mesenchymal transition

Smad2 and Smad3 as mediators of the response of adventitial fibroblasts induced by transforming growth factor β1

Review

Have you used Anti-Vimentin Rabbit Monoclonal Antibody?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality