Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 6 publication(s).
Anti-Vinculin/VCL Antibody Info At A Glance
|Product Name||Anti-Vinculin/VCL Antibody
See all Vinculin primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Vinculin/VCL detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. Vinculin/VCL information: Molecular Weight: 123799 MW; Subcellular Localization: Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions; Tissue Specificity: Metavinculin is muscle-specific.|
|Cite This Product||Anti-Vinculin/VCL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1781)|
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|Anti-Vinculin/VCL Antibody may replace the following items: sc 25336|sc 55465|sc 5573|sc 59803|sc 73264|sc 73614|sc 7648|sc 7649.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin(173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For VCL (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7414|
|Species Of This Entry||Human|
|Alternative Names||Vinculin|CMD1W; CMH15; metavinculin; MVCL; VCL; Vinculin|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Biotinylation; Methylation; Iodination|
|Subcellular Localization||Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.|
|Gene Location||10q22.2, on Chromasome 10, gene sequence: NC_000010.11 (73998116..74121363)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For VCL (Source: Uniprot.org, NCBI)
|Protein Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.|
|Tissue Specificity||Metavinculin is muscle-specific.|
|Interacting Proteins||ARPC2; CTNNA1; TEKT1|
|Related Diseases||Tissue Adhesions; Male Infertility; Infertility; Malignant Neoplasms; Neoplasms; Cell Invasion; Von Willebrand Disease; Cardiomyopathies; Thrombus; Neoplasm Metastasis; Headache; Edema; Dental Plaque; Cardiomyopathy, Dilated; Hypertrophy; Carcinoma; Hemorrhage; Arachnidism; Oligospermia|
|Related Pathways||Sperm Motility; Fertilization; Insemination; Acrosome Reaction; Cell Adhesion; Swimming; Cell Motility; Pathogenesis; Localization; Secretion; Platelet Aggregation; Spermatogenesis; Cell Proliferation; Transport; Cell Migration; Ovulation; Wound Healing; Sperm Capacitation; Regeneration; Hatching|
|Background||Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.|
|Scientific References||PMID: 2116004 by Weller P.A., et al. Complete sequence of human vinculin and assignment of the gene to chromosome 10.|
PMID: 8440716 by Moiseyeva E.P., et al. Organization of the human gene encoding the cytoskeletal protein vinculin and the sequence of the vinculin promoter.
Our Boster Quality Guarantee for Anti-Vinculin/VCL Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: U87 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HEPA Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: HT1080 Cell Lysate
IHC(P): Human Mammary Cancer Tissue
IHC(P): Rat Intestine Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 6
|Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.||PubMed ID 25888745|
|Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.||PubMed ID 25839041|
|Lyu Z, Wang H, Wang Y, Ding K, Liu H, Yuan L, Shi X, Wang M, Wang Y, Chen H. Nanoscale. 2014 Jun 21;6(12):6959-69. Doi: 10.1039/C4Nr01540A. Maintaining The Pluripotency Of Mouse Embryonic Stem Cells On Gold Nanoparticle Layers With Nanoscale But N...||PubMed ID 24839204|
|Wang H, Wang X, Qu J, Yue Q, Hu Y, Zhang H. J Cell Physiol. 2015 Nov;230(11):2728-42. Doi: 10.1002/Jcp.24997. Vegf Enhances The Migration Of Mscs In Neural Differentiation By Regulating Focal Adhesion Turnover.||PubMed ID 25820249|
|Lyu J, Hu Y, Xu X, Zhang H. J Cell Biochem. 2013 Aug;114(8):1744-59. Doi: 10.1002/Jcb.24517. Dynamics Of Focal Adhesions And Reorganization Of F-Actin In Vegf-Stimulated Nscs Under Varying Differentiation States.||PubMed ID 23444112|
|Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells||PubMed ID 29250149|
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Note: sample size takes longer to process, average 5-7 days.
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