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Product Info Summary
| SKU: | PA1781 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Vinculin/VCL Antibody Picoband®
SKU/Catalog Number
PA1781
BA2934 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vinculin/VCL Antibody catalog # PA1781. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vinculin/VCL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1781)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1781 is reactive to VCL in Mouse
Observed Molecular Weight
34 kDa
Calculated molecular weight
123.8 kDa
Background of VCL
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1781 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Heart Tissue, Rat Brain Tissue, Rat Liver Tissue, U87 Cell, SMMC Cell, HEPA Cell, HELA Cell, HT1080 Cell
IHC: Human Mammary Cancer Tissue, Rat Intestine Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Vinculin antibody, PA1781, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: U87 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HEPA Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: HT1080 Cell Lysate
Click image to see more details
Anti-Vinculin antibody, PA1781, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Vinculin antibody, PA1781, IHC(P)
IHC(P): Rat Intestine Tissue
Specific Publications For Anti-Vinculin/VCL Antibody Picoband® (PA1781)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Vinculin/VCL Antibody Picoband®
Question
We have been able to see staining in mouse endothelial cell. Any tips? Is anti-Vinculin/VCL antibody supposed to stain endothelial cell positively?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
From what I have seen in literature endothelial cell does express VCL. From what I have seen in Uniprot.org, VCL is expressed in myometrium, endothelial cell, retina, prostate, fetal brain cortex, uterus, platelet, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348
Boster Scientific Support
Answered: 2020-05-07
Question
I was wanting to use your anti-Vinculin/VCL antibody for IHC for mouse endothelial cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse endothelial cell identification?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, PA1781 anti-Vinculin/VCL antibody has been validated for IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse endothelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-30
Question
I am interested in to test anti-Vinculin/VCL antibody PA1781 on mouse endothelial cell for research purposes, then I may be interested in using anti-Vinculin/VCL antibody PA1781 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The products we sell, including anti-Vinculin/VCL antibody PA1781, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-17
Question
Here is the WB image, lot number and protocol we used for endothelial cell using anti-Vinculin/VCL antibody PA1781. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
I see that the anti-Vinculin/VCL antibody PA1781 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-14
Question
you antibody using your anti-Vinculin/VCL antibody for apical junction assembly studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We appreciate your inquiry. This PA1781 anti-Vinculin/VCL antibody is validated on rat heart tissue, tissue lysate, brain tissue, liver tissue, u87 cell lysate, smmc cell lysate, hepa cell lysate, hela cell lysate, mammary cancer tissue, intestine tissue. It is guaranteed to work for IHC, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-31
Question
Is a blocking peptide available for product anti-Vinculin/VCL antibody (PA1781)?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We do provide the blocking peptide for product anti-Vinculin/VCL antibody (PA1781). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-27
Question
Is this PA1781 anti-Vinculin/VCL antibody reactive to the isotypes of VCL?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The immunogen of PA1781 anti-Vinculin/VCL antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin(173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-05
Question
Does anti-Vinculin/VCL antibody PA1781 work on goat IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Our lab technicians have not validated anti-Vinculin/VCL antibody PA1781 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Vinculin/VCL antibody PA1781 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endothelial cell using anti-Vinculin/VCL antibody PA1781. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-25
Question
My team were well pleased with the WB result of your anti-Vinculin/VCL antibody. However we have observed positive staining in myometrium cell membrane using this antibody. Is that expected? Could you tell me where is VCL supposed to be expressed?
D. Jha
Verified customer
Asked: 2018-04-03
Answer
Based on literature, myometrium does express VCL. Generally VCL expresses in cell membrane. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348
Boster Scientific Support
Answered: 2018-04-03
Question
Is there a BSA free version of anti-Vinculin/VCL antibody PA1781 available?
E. Carter
Verified customer
Asked: 2016-05-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vinculin/VCL antibody PA1781 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-03
Question
We purchased anti-Vinculin/VCL antibody for WB on endothelial cell in the past. I am using mouse, and I plan to use the antibody for IHC next. We need examining endothelial cell as well as fetal brain cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
N. Johnson
Verified customer
Asked: 2015-07-16
Answer
I have checked the website and datasheets of our anti-Vinculin/VCL antibody and it appears that PA1781 has been tested on mouse in both WB and IHC. Thus PA1781 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-07-16
Question
We are currently using anti-Vinculin/VCL antibody PA1781 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on monkey tissues as well?
C. Banerjee
Verified customer
Asked: 2015-01-22
Answer
The anti-Vinculin/VCL antibody (PA1781) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-22
Question
Does PA1781 anti-Vinculin/VCL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Bhatt
Verified customer
Asked: 2014-05-07
Answer
It shows on the product datasheet, PA1781 anti-Vinculin/VCL antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-05-07
Question
My question regarding product PA1781, anti-Vinculin/VCL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Roberts
Verified customer
Asked: 2014-02-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1781 anti-Vinculin/VCL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-24
Question
Would anti-Vinculin/VCL antibody PA1781 work for IHC with endothelial cell?
A. Moore
Verified customer
Asked: 2013-03-12
Answer
According to the expression profile of endothelial cell, VCL is highly expressed in endothelial cell. So, it is likely that anti-Vinculin/VCL antibody PA1781 will work for IHC with endothelial cell.
Boster Scientific Support
Answered: 2013-03-12
