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Product Info Summary
| SKU: | A09012-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-XPLN ARHGEF3 Antibody
SKU/Catalog Number
A09012-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-XPLN ARHGEF3 Antibody catalog # A09012-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XPLN ARHGEF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09012-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ARHGEF3. AA range:456-505
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09012-1 is reactive to ARHGEF3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09012-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC:1:50-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using XPLN Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from COLO cells, using ARHGEF3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using ARHGEF3 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-XPLN ARHGEF3 Antibody (A09012-1)
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Customer Q&As
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4 Customer Q&As for Anti-XPLN ARHGEF3 Antibody
Question
We are currently using anti-XPLN antibody A09012-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-XPLN antibody (A09012-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
Would A09012-1 anti-XPLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can see on the product datasheet, A09012-1 anti-XPLN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Would anti-XPLN antibody A09012-1 work on goat WB with testis?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Our lab technicians have not tested anti-XPLN antibody A09012-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-XPLN antibody A09012-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
We need to test anti-XPLN antibody A09012-1 on mouse testis for research purposes, then I may be interested in using anti-XPLN antibody A09012-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
The products we sell, including anti-XPLN antibody A09012-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-07
