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Product Info Summary
| SKU: | A09012-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-XPLN ARHGEF3 Antibody
SKU/Catalog Number
A09012-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-XPLN ARHGEF3 Antibody catalog # A09012-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XPLN ARHGEF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09012-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
3D5B1
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human XPLN. at AA rangle: 430-510
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09012-1 is reactive to ARHGEF3 in Human, Mouse, Rat
Applications
A09012-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.783kDa
Background of ARHGEF3
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using XPLN Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF3 (Source: Uniprot.org, NCBI)
Gene Name
ARHGEF3
Full Name
Rho guanine nucleotide exchange factor 3
Weight
59.783kDa
Alternative Names
DKFZP434F2429; Exchange factor found in platelets and leukemic and neuronal tissues; FLJ98126; MGC118905; Rho guanine nucleotide exchange factor (GEF) 3; rho guanine nucleotide exchange factor 3,59.8 kDA protein; RhoGEF protein; XPLNXPLN ARHGEF3 GEF3, STA3, XPLN Rho guanine nucleotide exchange factor 3 rho guanine nucleotide exchange factor 3|59.8 kDA protein|Rho guanine nucleotide exchange factor (GEF) 3|RhoGEF protein|exchange factor found in platelets and leukemic and neuronal tissues, XPLN
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGEF3, check out the ARHGEF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGEF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XPLN ARHGEF3 Antibody (A09012-1)
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No publications found for A09012-1
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Customer Q&As
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4 Customer Q&As for Anti-XPLN ARHGEF3 Antibody
Question
We are currently using anti-XPLN antibody A09012-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-XPLN antibody (A09012-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
Would A09012-1 anti-XPLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can see on the product datasheet, A09012-1 anti-XPLN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Would anti-XPLN antibody A09012-1 work on goat WB with testis?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Our lab technicians have not tested anti-XPLN antibody A09012-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-XPLN antibody A09012-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
We need to test anti-XPLN antibody A09012-1 on mouse testis for research purposes, then I may be interested in using anti-XPLN antibody A09012-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
The products we sell, including anti-XPLN antibody A09012-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-07
