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Product Info Summary
| SKU: | A02797 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-5'-3' exoribonuclease 2 XRN2 Antibody
SKU/Catalog Number
A02797
Size
100μl
Form
Liquid
Description
Boster Bio Anti-5'-3' exoribonuclease 2 XRN2 Antibody catalog # A02797. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-5'-3' exoribonuclease 2 XRN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02797)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
4E7E1
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human XRN2. AA range:81-130
Reactive Species
A02797 is reactive to XRN2 in Human, Monkey, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
108.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02797 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using XRN2 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COS7 cells, using XRN2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using XRN2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-5'-3' exoribonuclease 2 XRN2 Antibody (A02797)
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Customer Q&As
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6 Customer Q&As for Anti-5'-3' exoribonuclease 2 XRN2 Antibody
Question
Would anti-XRN2 antibody A02797 work on horse WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Our lab technicians have not tested anti-XRN2 antibody A02797 on horse. You can run a BLAST between horse and the immunogen sequence of anti-XRN2 antibody A02797 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-08
Question
We are currently using anti-XRN2 antibody A02797 for monkey tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The anti-XRN2 antibody (A02797) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-28
Question
Does A02797 anti-XRN2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
It shows on the product datasheet, A02797 anti-XRN2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-13
Question
My question regarding product A02797, anti-XRN2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Parker
Verified customer
Asked: 2018-12-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02797 anti-XRN2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-03
Question
Is this A02797 anti-XRN2 antibody reactive to the isotypes of XRN2?
L. Wu
Verified customer
Asked: 2018-11-20
Answer
The immunogen of A02797 anti-XRN2 antibody is Synthesized peptide derived from the N-terminal region of human XRN2. at AA rangle: 50-130. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-20
Question
I see that the anti-XRN2 antibody A02797 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-11-24
