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Product Info Summary
| SKU: | A06831 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-ZBTB7B Antibody (C-term)
SKU/Catalog Number
A06831
Size
80 µl
Description
Boster Bio Anti-ZBTB7B Antibody (C-term) (Catalog # A06831). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ZBTB7B Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06831)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-469 amino acids from the C-terminal region of human ZBTB7B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06831 is reactive to ZBTB7B in Human
Reconstitution
Calculated molecular weight
58027 Da
Background of Th-POK
ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06831 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.
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All lanes : Anti-ZBTB7B Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 58 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For ZBTB7B (Source: Uniprot.org, NCBI)
Gene Name
ZBTB7B
Full Name
Zinc finger and BTB domain-containing protein 7B
Weight
58027 Da
Alternative Names
Zinc finger and BTB domain-containing protein 7B, Krueppel-related zinc finger protein cKrox, hcKrox, T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 15, Zinc finger protein 67 homolog, Zfp-67, Zinc finger protein 857B, Zinc finger protein Th-POK, ZBTB7B, ZBTB15, ZFP67, ZNF857B Zbtb7b|Thp, Thpok, Zfp6, Zfp67, c-Kr, c-Krox|zinc finger and BTB domain containing 7B|zinc finger and BTB domain-containing protein 7B|T-helper-inducing POZ/Krueppel-like factor|Vzinc finger and BTB domain containing 7B|krueppel-related zinc finger protein cKrox|zinc finger protein 67|zinc finger protein Th-POK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZBTB7B, check out the ZBTB7B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZBTB7B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZBTB7B Antibody (C-term) (A06831)
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