This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Arachidonate 5-lipoxygenase.
Human | |
---|---|
Gene Name: | ALOX5 |
Uniprot: | P09917 |
Entrez: | 240 |
Belongs to: |
---|
lipoxygenase family |
5Lipoxygenase; 5-Lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 1.13.11.34; leukotriene A4 synthase; LOG5; LOX5A; MGC163204
Mass (kDA):
77.983 kDA
Human | |
---|---|
Location: | 10q11.21 |
Sequence: | 10; NC_000010.11 (45374166..45446121) |
Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Antibody against 5 lipoxygenase/ALOX5 is available from Boster Bio. We will be discussing the Benefits, Validation, and Applications of this Marker. Boster is an antibody manufacturer, and its products are validated on multiple platforms using known positive and negative samples. Boster recognizes the contributions made by scientists around the globe by awarding product credits for the first reviewers.
Transcription is the process that allows cells to express the ALOX5 genes. Patients with a sequence variant ALOX5 will experience reduced ALOX5 production, which could lead to a decreased response for drugs that target this pathway. It is possible to alter ALOX5 expression by adjusting the promoter or gene sequence. Hence, researchers can use Boster Bio Antibody to test for the presence of a specific mutation in a patient's body.
Boster Bio has an Anti-5 Lipoxygenase/ALOX5 anti-ALOX5 antibody which can be used to inhibit the ALOX5 enzyme's activity in human cells. The company's antibodies have high sensitivity and are validated for flow-cytometry, WB, IHC and WB. They can detect biomarkers down to the picogram level and are tested against 250 tissue panels. Boster Bio offers a variety of immunological reagents, including the ALOX5 antibody as well as other ELISA kits.
FLAP (Flap) is a protein that regulates the activity and function of the 5-lipoxygenase. The enzyme is responsible to catalyze the oxidation and formation of arachidonic, which is a precursor for leukotrienes. Mice lacking this enzyme exhibit anxiety-like behaviors. This enzyme is an active player in Alzheimer’s disease pathogenesis.
Studies have shown that AA 15-lipoxygenating ALOX15 orthologs are more effective at controlling inflammatory responses in mammals than their AA-15-lipoxygenase orthologs. This evolutionary switch has been supported by in vitro activity and the Triad Concept. ALOX15 orthologs are high in biosynthesis in mammals, which is supported by the discovery ALOX15 within the nine-banded armadillo.
The Boster Kit was used for the extraction of proteins from tumour tissues and U937 cells. GenScript and Bosterbio provided antibodies for the generation of anti-Gcet1 antigen that was raised against Cyp2c44. The functional homologue of human Cyp2c9. The anti–Gcet1 antibody inhibits endothelial tissue proliferation and downregulation Cyp2c44 decreases tubular growth.
The ALOX15 orthologs have been functionally characterized in over 70 mammals. 95% of mammalian ALOX15 homologs can be predicted to have the same specificity, AA 12-lipoxygenating and other enzyme classification criteria using the Triad Concept. Therefore, the Triad Concept is the most logical explanation of the evolution of the AA 15 and ALOX15 enzymes.
The ALOX5 gene encodes an iron-containing dioxygenase that is non-heme. This enzyme catalyzes the first step in leukotriene biosynthesis and is known to be involved in inflammatory processes. ALOX5 converts arachidonate into the unstable intermediate LTA4 in just two steps. The final product of arachidonate synthesis is cysteinyl-leukotriene, which is a key mediator in inflammatory diseases, including asthma.
While previous research has implicated ALOX5 for the development of chronic myeloidleukemia in mouse stem cell cells, its importance in human disease is still unknown. The current study used cell surface flow cytometry analysis to determine functional ALOX5 levels. It also used a TaqMan expression assay and LTB4 ELISA. The study revealed that the gene is expressed even in low levels of ALOX5 expression on leukemic cells.
ALOX5 genes are important mediators of inflammation. This enzyme is a member of the lipoxygenase group and plays a crucial role in the biosynthesis leukotrienes. They are involved in normal host defense and in disease states, such as chronic inflammation. The ALOX5 Gene is well-known for its role in both biosynthesis of proinflammatory and anti-inflammatory leukotrienes. An imbalance of anti-inflammatory and proinflammatory cytokines during inflammation can lead to preeclampsia.
Alox-5 is also implicated in the differentiation process of some cell lines including K562 cells and Lucena cells. One study found that Alox-5 expression was significantly higher in non-MDR K562 cells than in MDR Lucena cells. However, the MDR Lucena line had a higher number megakaryocytes. PMA had no effect on Alox-5 expression. It was a key determinant of the outcome of the experiment.
The ALOX5 promoter has four tandem repeats of five nucleotides. These repeats help to increase the phenotypic variation of patients. Pharmacogenetic studies should therefore focus on the ALOX5 genes. It has been shown that ALOX5 promoter polymorphisms are associated with higher incidence of cardiovascular disease in African Americans compared to whites.
ALOX5 also plays a role in the severity of COVID-19 in individuals with diabetes. It is positively associated to genes involved inflammation and infection. ALOX5 expression is positively correlated with LTB4 and ACE2 genes. When ALOX5 is elevated, mononuclear cells are recruited to the lungs and play a role in regulating the level of infection and healing. They can also cause tissue destruction.
PMID: 3422434 by Dixon R.A.F., et al. Cloning of the cDNA for human 5-lipoxygenase.
PMID: 2829172 by Matsumoto T., et al. Molecular cloning and amino acid sequence of human 5-lipoxygenase.