ALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA Kit

5-Lipoxygenase ELISA kit, phospho-specific, cell-based

The ALOX5 (Phospho-Ser271) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ALOX5 (Phospho-Ser271) protein expression profile in cells. The kit can be used for measuring the relative amounts of ALOX5 (Phospho-Ser271) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ALOX5 (Phospho-Ser271).

Product Info Summary

SKU: EKC2187
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

ALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA Kit

See all 5-Lipoxygenase products

SKU/Catalog Number

EKC2187

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The ALOX5 (Phospho-Ser271) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ALOX5 (Phospho-Ser271) protein expression profile in cells. The kit can be used for measuring the relative amounts of ALOX5 (Phospho-Ser271) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ALOX5 (Phospho-Ser271).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

ALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2187)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2187 is reactive to ALOX5 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2187 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-ALOX5 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-ALOX5 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Gene/Protein Information For ALOX5 (Source: Uniprot.Org, NCBI)

Gene Name

ALOX5

Full Name

Arachidonate 5-lipoxygenase

Weight

77.983kDa

Superfamily

lipoxygenase family

Alternative Names

5Lipoxygenase; 5-Lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 1.13.11.34; leukotriene A4 synthase; LOG5; LOX5A; MGC163204 ALOX5 5-LO, 5-LOX, 5LPG, LOG5 arachidonate 5-lipoxygenase polyunsaturated fatty acid 5-lipoxygenase|LOX-5|arachidonic acid 5-lipoxygenase|leukotriene A4 synthase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ALOX5, check out the ALOX5 Infographic

ALOX5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ALOX5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EKC2187

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used ALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review ALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA Kit

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for ALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA Kit

$540
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
Eddy test
In stock
Order Product
EKC2187
$540.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.