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Product Info Summary
| SKU: | M00847 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-5 Lipoxygenase ALOX5 Monoclonal Antibody
SKU/Catalog Number
M00847
BM5522 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-5 Lipoxygenase ALOX5 Monoclonal Antibody catalog # M00847. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-5 Lipoxygenase ALOX5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00847)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AECE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 5 Lipoxygenase
Reactive Species
M00847 is reactive to ALOX5 in Human, Mouse, Rat
Observed Molecular Weight
78 kDa
Calculated molecular weight
78.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00847 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human K562 whole cell, human RT4 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ALOX5 using anti-ALOX5 antibody (M00847).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX5 antigen affinity purified monoclonal antibody (Catalog # M00847) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALOX5 at approximately 78 kDa. The expected band size for ALOX5 is at 78 kDa.
Specific Publications For Anti-5 Lipoxygenase ALOX5 Monoclonal Antibody (M00847)
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Customer Q&As
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7 Customer Q&As for Anti-5 Lipoxygenase ALOX5 Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for brain using anti-5 Lipoxygenase Monoclonal antibody M00847. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
Does anti-5 Lipoxygenase Monoclonal antibody M00847 work on pig WB with brain?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not validated anti-5 Lipoxygenase Monoclonal antibody M00847 on pig. You can run a BLAST between pig and the immunogen sequence of anti-5 Lipoxygenase Monoclonal antibody M00847 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
I have a question about product M00847, anti-5 Lipoxygenase Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00847 anti-5 Lipoxygenase Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-11
Question
We need to test anti-5 Lipoxygenase Monoclonal antibody M00847 on rat brain for research purposes, then I may be interested in using anti-5 Lipoxygenase Monoclonal antibody M00847 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The products we sell, including anti-5 Lipoxygenase Monoclonal antibody M00847, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-08
Question
Is a blocking peptide available for product anti-5 Lipoxygenase Monoclonal antibody (M00847)?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We do provide the blocking peptide for product anti-5 Lipoxygenase Monoclonal antibody (M00847). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-31
Question
Is there a BSA free version of anti-5 Lipoxygenase Monoclonal antibody M00847 available?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-5 Lipoxygenase Monoclonal antibody M00847 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-01
Question
Is this M00847 anti-5 Lipoxygenase Monoclonal antibody reactive to the isotypes of ALOX5?
W. Li
Verified customer
Asked: 2019-01-08
Answer
The immunogen of M00847 anti-5 Lipoxygenase Monoclonal antibody is A synthesized peptide derived from human 5 Lipoxygenase Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-08
