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Product Info Summary
| SKU: | LS000240 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of arachidonate 5-lipoxygenase (ALOX5) |
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Product info
Product Name
5 Lipoxygenase (ALOX5) (NM_000698) Human Over-expression Lysate
See all 5-Lipoxygenase products
SKU/Catalog Number
LS000240
Size
100 µg
Description
Transient overexpression lysate of arachidonate 5-lipoxygenase (ALOX5)
Storage & Handling
Cite This Product
5 Lipoxygenase (ALOX5) (NM_000698) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000240)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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5 Lipoxygenase (ALOX5) Human Over-expression Lysates NM_000698.
Western blot validation of overexpression lysate (LS000240) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ALOX5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ALOX5 (Source: Uniprot.org, NCBI)
Gene Name
ALOX5
Full Name
Arachidonate 5-lipoxygenase
Weight
77.983kDa
Superfamily
lipoxygenase family
Alternative Names
5Lipoxygenase; 5-Lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 1.13.11.34; leukotriene A4 synthase; LOG5; LOX5A; MGC163204 ALOX5 5-LO, 5-LOX, 5LPG, LOG5 arachidonate 5-lipoxygenase polyunsaturated fatty acid 5-lipoxygenase|LOX-5|arachidonic acid 5-lipoxygenase|leukotriene A4 synthase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ALOX5, check out the ALOX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALOX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For 5 Lipoxygenase (ALOX5) (NM_000698) Human Over-expression Lysate (LS000240)
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