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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-CD105/ENG Antibody Picoband™
Boster Bio Anti-CD105/ENG Antibody Picoband™ catalog # A02997-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD105/ENG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02997-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence in the middle region of human CD105 (258-297aa YVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQ), different from the related mouse sequence by twelve amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A02997-1 is reactive to ENG in Human, Mouse, Rat
A02997-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD105 using anti-CD105 antibody (A02997-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: HELA whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105 antigen affinity purified polyclonal antibody (Catalog # A02997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD105 at approximately 95KD. The expected band size for CD105 is at 81KD.
Gene/Protein Information For ENG (Source: Uniprot.Org, NCBI)
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1 ENG END, HHT1, ORW1 endoglin endoglin|CD105 antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ENG, check out the ENG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ENG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A02997-1 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
VEGF promotes cartilage angiogenesis by phospho-ERK1/2 activation of Dll4 signaling in temporomandibular joint osteoarthritis caused by chronic sleep %u2026
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy
Overexpression of transcription factor Foxa2 and Hnf1? induced rat bone mesenchymal stem cells into hepatocytes
Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes
Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface
Ren Sx, Ren Zj, Zhao My, Wang Xb, Zuo Sg, Yu F. Acta Pharmacol Sin. 2009 May;30(5):637-45. Doi: 10.1038/Aps.2009.44. Antitumor Activity Of Endogenous Mflt4 Displayed On A T4 Phage Nanoparticle Surface.
Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.
Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z. Int J Biol Sci. 2013 Nov 21;9(10):1089-98. Doi: 10.7150/Ijbs.7367. Ecollection 2013. Genistein Promotion Of Osteogenic Differentiation Through Bmp2/Smad5/Runx2 Signaling.
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