Product Info Summary
SKU: | FEK0644 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit
See all Endoglin/CD105 products
SKU/Catalog Number
FEK0644
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD105 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0644)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: E26-G586
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0644 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0644 is reactive to ENG in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK0644 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endoglin/CD105
CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0644-CAP | 96-well plate precoated with anti-Human Endoglin/CD105 antibody | 1 |
EK0644-ST | lyophilized recombinant Human Endoglin/CD105 standard | 10 ng/tube |
FEK0644-DA | Biotinylated anti-Human Endoglin/CD105 detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.011 | 0.102 | 0.258 | 0.471 | 0.766 | 1.312 | 1.658 | 2.022 |
Data Example Images
Click image to see more details
Human CD105 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 312 | 1384 | 4821 | 316 | 1275 | 4955 |
Standard deviation | 16.22 | 74.73 | 318.18 | 17.38 | 72.67 | 386.49 |
CV (%) | 5.2% | 5.4% | 6.6% | 5.5% | 5.7% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Endoglin/CD105 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 312 | 285 | 280 | 306 | 295 | 13.53 | 4.5% |
Sample 2 | 1384 | 1301 | 1412 | 1238 | 1333 | 68.71 | 5.1% |
Sample 3 | 4821 | 5271 | 4779 | 5474 | 5086 | 295.48 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For ENG (Source: Uniprot.Org, NCBI)
Uniprot ID
P17813
Gene ID
2022
Gene Name
ENG
Full Name
Endoglin
Weight
70.578kDa
Alternative Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1 ENG END, HHT1, ORW1 endoglin endoglin|CD105 antigen
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ENG, check out the ENG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ENG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit (FEK0644)
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Customer Q&As
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1 Customer Q&As for Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit
Question
I purchased your product Human CD105 PicoKine and I would like to know the best anti-coagulant for collecting plasma samples.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The Human CD105 PicoKine has been validated with plasma and is suitable with EDTA, heparin and citrate anti-coagulants.
Boster Scientific Support
Answered: 2019-07-19