Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit

Endoglin/CD105 ELISA kit, 120-minute protocol

Human CD105 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Product Info Summary

SKU: FEK0644
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 120 Minutes

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Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit

See all Endoglin/CD105 products

SKU/Catalog Number

FEK0644

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD105 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0644)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: E26-G586

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0644 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0644 is reactive to ENG in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0644 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Endoglin/CD105

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

Kit Components

Catalog number Description Quantity
FEK0644-CAP 96-well plate precoated with anti-Human Endoglin/CD105 antibody 1
EK0644-ST lyophilized recombinant Human Endoglin/CD105 standard 10 ng/tube
FEK0644-DA Biotinylated anti-Human Endoglin/CD105 detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0110.1020.2580.4710.7661.3121.6582.022

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3121384482131612754955
Standard deviation16.2274.73318.1817.3872.67386.49
CV (%)5.2%5.4%6.6%5.5%5.7%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Endoglin/CD105 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131228528030629513.534.5%
Sample 21384130114121238133368.715.1%
Sample 348215271477954745086295.485.8%
*number of samples for each test n=16.

Gene/Protein Information For ENG (Source: Uniprot.Org, NCBI)

Uniprot ID

P17813

Gene ID

2022

Gene Name

ENG

Full Name

Endoglin

Weight

70.578kDa

Alternative Names

CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1 ENG END, HHT1, ORW1 endoglin endoglin|CD105 antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ENG, check out the ENG Infographic

ENG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ENG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit

Question

I purchased your product Human CD105 PicoKine and I would like to know the best anti-coagulant for collecting plasma samples.

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

The Human CD105 PicoKine has been validated with plasma and is suitable with EDTA, heparin and citrate anti-coagulants.

Boster Scientific Support

Answered: 2019-07-19

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