Anti-Hu CD105 Purified ENG Monoclonal Antibody

Product Name

Anti-Hu CD105 Purified ENG Monoclonal Antibody

View all ENG Antibodies

SKU/Catalog Number

M02997-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD105 Purified ENG Monoclonal Antibody (Catalog# M02997-2). Tested in Flow Cytometry, WB, IHC-F, ICC application(s). This antibody reacts with Pig, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD105 Purified ENG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02997-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-229

Isotype

Mouse IgG2a

Immunogen

Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA. The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02997-2 is reactive to ENG in Human, Pig

Observed Molecular Weight

42 kDa

Calculated molecular weight

70.6 kDa

Background of ENG

CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02997-2 is guaranteed for Flow Cytometry, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 2-6 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line.
Immunohistochemistry (frozen sections): 1:200, acetone fixation.
Western blotting: Non-reducing conditions.

Validation Images & Assay Conditions

See full target information ENG

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