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12 Citations 7 Q&As
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Facts about Nuclear factor NF-kappa-B p105 subunit.
The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. NF-kappa-B is controlled by several mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors.
Human | |
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Gene Name: | NFKB1 |
Uniprot: | P19838 |
Entrez: | 4790 |
Belongs to: |
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No superfamily |
DKFZp686C01211; DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; NF-kappaB; NF-kappa-B; NF-kappabeta; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151; p105; p50
Mass (kDA):
105.356 kDA
Human | |
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Location: | 4q24 |
Sequence: | 4; NC_000004.12 (102501266..102617302) |
Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
For many years, researchers and scientists have been using the NFKB1 marker in their research to determine the degree of a particular gene's expression in the body. The new ELISA kit created by Boster Bio has helped researchers discover a new method to use the NFKB1 marker to determine the gene expression of many cells. The NFKB1 marker is the first expression level to be identified by the International Organization for Genomic Regulation (ISO) as a biomarker of tumor cells.
Researchers can observe the activity of their cells by using secondary and primary antibodies against NFKB1 the transcription element. Primary antibodies, like monoclonal antibody from mice against FAS are highly specific and can be used to detect protein fusions. For flow cytometry, secondary antibodies, such as those against CD45 from mice can also be employed. These antibodies have been quantitatively validated by testing them against known amounts of Recombinant proteins. Furthermore, these antibodies have high affinity for the target protein, which results in reliable results.
Immunocytochemical staining of cells was performed as described previously. Cells were fixed in the presence of 4% PFA, then permeated using 0.5 percent Triton X-100 PBS. After blocking, cells were incubated with primary antibodies and secondary antibodies at a concentration of 1:100. The incubation was for 60 minutes. Secondary antibodies were used to stain NFKB1 microplates coated with protein.
Boster Bio's research kit NFKB1, called nuclear factor kappa B is an excellent choice for life science research. These reagents are able to detect NFKB1 in a variety of cell types and offer precise results. You can choose from a wide variety of options depending on the project's needs.
One of the most recent tools for research, Boster Bio's PICOKINE (tm) ELISA kits for the human NFKB1 marker are the first to provide an phosphorylated version of the protein. This protein is present in plasma membranes and serves as a marker for nitric oxide production. Researchers can make use of PICOKINE ELISA kits to analyze the protein expression of NFKB1 in cell cultures. These kits are also approved for use in flow cytometry, IHC, WB and WB.
The PicoKINE(tm) kits for ELISA employ a unique method that minimizes non-specific binding, and increases the capture antibodies. The result is high sensitivity and a low background, making it an extremely accurate marker. The PICOKINE(tm) ELISA kits have been thoroughly validated in several samples. People with limited funds can select the standard or deluxe kits.
PMID: 2203531 by Kieran M., et al. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.
PMID: 2234062 by Bours V., et al. Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs.
*Showing only the more recent 20. More publications can be found for each product on its corresponding product page