Anti-NFKB1 Antibody

NFKB1 antibody

Boster Bio Anti-NFKB1 Antibody (Catalog # M00283-2). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.

Product Info Summary

SKU: M00283-2
Size: 80 µl
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC-P, WB

Product Name

Anti-NFKB1 Antibody

View all NFKB1 Antibodies

SKU/Catalog Number

M00283-2

Size

80 µl

Description

Boster Bio Anti-NFKB1 Antibody (Catalog # M00283-2). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-NFKB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00283-2)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1298CT792.105.117.133

Isotype

IgG1,κ

Immunogen

This antibody is generated from a mouse immunized with a recombinant protein from human NFKB1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00283-2 is reactive to NFKB1 in Human

Applications

M00283-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

105.356kDa

Background of NFKB1

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:25
FC: 1:25

Validation Images & Assay Conditions

Gene/Protein Information For NFKB1 (Source: Uniprot.org, NCBI)

Gene Name

NFKB1

Full Name

Nuclear factor NF-kappa-B p105 subunit

Weight

105.356kDa

Alternative Names

DKFZp686C01211; DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; NF-kappaB; NF-kappa-B; NF-kappabeta; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151; p105; p50 NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFKB1, check out the NFKB1 Infographic

NFKB1 infographic

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In this infographic, you will see the following information for NFKB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00283-2-80ul

80 µl

$298
M00283-2-400ul

400 µl

$460

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