Click image to see more details
Product Info Summary
| SKU: | AZA0A8M9QGX8 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband®
SKU/Catalog Number
AZA0A8M9QGX8
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband® catalog # AZA0A8M9QGX8. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M9QGX8)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish NFKB1 recombinant protein (Position: E10-K364).
Reactive Species
AZA0A8M9QGX8 is reactive to NFKB1 in Zebrafish
Background of NFKB1
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in anatomical structure morphogenesis; epithelium development; and nervous system development. Predicted to act upstream of or within regulation of DNA-templated transcription. Predicted to be located in cytoplasm. Predicted to be part of NF-kappaB p50/p65 complex. Is expressed in brain and retina. Human ortholog(s) of this gene implicated in several diseases, including HTLV-1-associated myelopathy/tropical spastic paraparesis; carcinoma (multiple); common variable immunodeficiency 12; melanoma; and prostate cancer. Orthologous to human NFKB1 (nuclear factor kappa B subunit 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A8M9QGX8 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of NFKB1 using anti-NFKB1 antibody (AZA0A8M9QGX8).
NFKB1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFKB1 Antibody (AZA0A8M9QGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Western blot analysis of NFKB1 using anti-NFKB1 antibody (AZA0A8M9QGX8).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKB1 antigen affinity purified polyclonal antibody (AZA0A8M9QGX8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFKB1 at approximately 55 kDa. The expected band size for NFKB1 is at 50 kDa.
Specific Publications For Anti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband® (AZA0A8M9QGX8)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
