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Product Info Summary
| SKU: | A00283S932 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-N kappa-p105 (S932) NFKB1 Antibody
SKU/Catalog Number
A00283S932
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-N kappa-p105 (S932) NFKB1 Antibody catalog # A00283S932. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-N kappa-p105 (S932) NFKB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00283S932)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser932. AA range:899-948
Reactive Species
A00283S932 is reactive to NFKB1 in Human, Mouse, Rat
Calculated molecular weight
105.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00283S932 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells treated with CA+TNF 20ng/ml 10', using NF-kappaB p105/p50 (Phospho-Ser932) Antibody. The lane on the left is blocked with the phospho peptide.
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Western Blot analysis of 1 A431 treated with LPS, 2 A431, using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p105/p50 (Phospho-Ser932) Antibody
Specific Publications For Anti-Phospho-N kappa-p105 (S932) NFKB1 Antibody (A00283S932)
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