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- Table of Contents
NFKB1 antibody (M00283) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Immunohistochemical analysis of paraffin-embedded human kidney, using NF-κB p105/p50 Antibody.
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Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody
NFKB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
NFKB1 antibody was positively detected by WB in:
human RT4 whole cell, human A431 whole cell, human SiHa whole cell, human CACO-2 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse NIH/3T3 whole cell, mouse RAW264.7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NFKB1 antibody was positively detected by IHC in:
human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody
View all NFKB1 Antibodies
View all NFKB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ID-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-κB (p105/p50)
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50, 105 kDa
Calculated Molecular Weight
105.4 kDa
Cite This Product
Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00283)
Protocols
Western Blot protocol for NFKB1 antibody
Immunohistochemistry (IHC) protocol for NFKB1 antibody
Publications
Application:
Species:
Emerging Alternaria Toxins Drive LX-2 Cells Transdifferentiation into Myofibroblasts for Liver Fibrosis and CotA Detoxification
Published: 2026-03-30
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 6.20
Species: Human
PMID: 41906758
The H4R antagonist, JNJ-7777120 treatments ameliorate mild traumatic brain injury by reducing oxidative damage, inflammatory and apoptotic responses through blockage of the ERK1/2/NF-κB pathway in a rat model
Published: 2024-12-26
Journal: EXPERIMENTAL NEUROLOGY
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 39732275
Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis.
Published: 2024-09-19
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.70
Species: Human
PMID: 39341126
Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells
Published: 2023-01-02
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.80
Species: Human
PMID: 36603687
Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora
Published: 2022-09-14
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 5.22
Species: Mouse
MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway
Published: 2022-06-21
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB, IF
Impact Factor: 5.88
Species: Human
PMID: 35730614
Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway
Published: 2020-08-30
Journal: MOLECULAR AND CELLULAR ENDOCRINOLOGY
Application: WB
Impact Factor: 3.87
Species: Rat
PMID: 32877753
Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats
Published: 2019-02-08
Journal: JOURNAL OF MOLECULAR NEUROSCIENCE
Application: WB
Impact Factor: 2.58
Species: Rat
PMID: 30734208
Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress
Published: 2018-04-04
Journal: Gastroenterology Research and Practice
Application: WB
Impact Factor: 1.86
Species: Rat
PMID: 29849603
HMGB1, TGF‑β and NF‑κB are associated with chronic allograft nephropathy
Published: 2017-10-17
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 1.26
Species: Human
PMID: 29285170
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway
Published: 2014-03-18
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 2.11
Species: Human
PMID: 24721321
Review
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Product Promise
We promise Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00283
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026