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Product Info Summary
| SKU: | M00283 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00283
BM3946 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody catalog # M00283. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00283)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ID-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-κB (p105/p50)
Reactive Species
M00283 is reactive to NFKB1 in Human, Mouse, Rat
Observed Molecular Weight
50, 105 kDa
Calculated molecular weight
105.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00283 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human RT4 whole cell, human A431 whole cell, human SiHa whole cell, human CACO-2 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse NIH/3T3 whole cell, mouse RAW264.7 whole cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of NF-κB p105/p50 using anti-NF-κB p105/p50 antibody (M00283).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-κB p105/p50 antigen affinity purified monoclonal antibody (M00283) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-κB p105/p50 at approximately 50, 105 kDa. The expected band size for NF-κB p105/p50 is at 50, 105 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using NF-κB p105/p50 Antibody.
Specific Publications For Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody (M00283)
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