Anti-NFkB/NFKB1 p105/p50 Antibody Picoband®

Product Name

Anti-NFkB/NFKB1 p105/p50 Antibody Picoband®

View all NFKB1 Antibodies

SKU/Catalog Number

PA2111-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NFkB/NFKB1 p105/p50 Antibody catalog # PA2111-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NFkB/NFKB1 p105/p50 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2111-1)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NFkB/NFKB1 p105/p50, different from the related rat sequence by one amino acid and from the related mouse sequence by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2111-1 is reactive to NFKB1 in Human, Mouse, Rat

Observed Molecular Weight

105 kDa

Calculated molecular weight

105.4 kDa

Background of NFKB1

Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2111-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: HELA Whole Cell, JURKAT Whole Cell

Validation Images & Assay Conditions

See full target information NFKB1

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