Product Info Summary
SKU: | LS001438 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (CSF2RA), transcript variant 1 |
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Product info
Product Name
GM CSF Receptor alpha (CSF2RA) (NM_006140) Human Over-expression Lysate
See all GM-CSFR alpha products
SKU/Catalog Number
LS001438
Size
100 µg
Description
Transient overexpression lysate of colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (CSF2RA), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
GM CSF Receptor alpha (CSF2RA) (NM_006140) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001438)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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GM CSF Receptor alpha (CSF2RA) Human Over-expression Lysates NM_001161529.
Western blot validation of overexpression lysate (LS001438) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSF2RA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
GM CSF Receptor alpha (CSF2RA) Human Over-expression Lysates NM_001161531.
Western blot validation of overexpression lysate (LS001438) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSF2RA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
GM CSF Receptor alpha (CSF2RA) Human Over-expression Lysates NM_006140.
Western blot validation of overexpression lysate (LS001438) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSF2RA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
GM CSF Receptor alpha (CSF2RA) Human Over-expression Lysates NM_172245.
Western blot validation of overexpression lysate (LS001438) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSF2RA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
GM CSF Receptor alpha (CSF2RA) Human Over-expression Lysates NM_172247.
Western blot validation of overexpression lysate (LS001438) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSF2RA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CSF2RA (Source: Uniprot.org, NCBI)
Gene Name
CSF2RA
Full Name
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
Weight
46.207kDa
Superfamily
type I cytokine receptor family
Alternative Names
CD116 antigen; CD116; CDw116; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage); CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF R alpha; GM-CSF receptor alpha subunit; GMCSFR alpha; GMCSFR; GM-CSFRa; GM-CSF-R-alpha; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838 CSF2RA CD116, CDw116, CSF2RX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMCSFR-alpha, GMR, GMR-alpha, SMDP4, alphaGMR, CSF2RA colony stimulating factor 2 receptor subunit alpha granulocyte-macrophage colony-stimulating factor receptor subunit alpha|CD116 |GM-CSF receptor alpha subunit|alpha-GM-CSF receptor|colony stimulating factor 2 receptor alpha subunit|colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)|granulocyte-macrophage colony-stimulating factor receptor alpha chain
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CSF2RA, check out the CSF2RA Infographic
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In this infographic, you will see the following information for CSF2RA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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