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Product Info Summary
| SKU: | M04432 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB, CyTOF |
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Product info
Product Name
Anti-Hu CD116 Purified CSF2RA Monoclonal Antibody
SKU/Catalog Number
M04432
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD116 Purified CSF2RA Monoclonal Antibody (Catalog# M04432). Tested in Flow Cytometry, IP, WB, IHC-F, MC (CyTOF) application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD116 Purified CSF2RA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04432)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
4H1
Isotype
Mouse IgG1 kappa
Immunogen
CD116-transfected COS cells. The mouse monoclonal antibody 4H1 recognizes an extracellular epitope of human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04432 is reactive to CSF2RA in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
46.2 kDa
Background of CSF2RA
CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5- receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04432 is guaranteed for Flow Cytometry, IP, IHC-F, WB, CyTOF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD116 (4H1) purified antibody (concentration in sample 3 µg/ml) GAM PE.
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD116 (4H1) purified antibody (concentration in sample 3 µg/ml) GAM PE.
Specific Publications For Anti-Hu CD116 Purified CSF2RA Monoclonal Antibody (M04432)
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