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Product Info Summary
| SKU: | FEK1811 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD164 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1811
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD164 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD164 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD164 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1811)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D24-D162
Sensitivity
<50 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1811 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1811 is reactive to CD164 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
FEK1811 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD164
Sialomucin core protein 24 also known as endolyn or CD164， is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1811-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1811-ST | Human CD164 Standard | 2 | 10 ng/tube |
| FEK1811-A | Human CD164 Antibody A | 1 | 3ml |
| FEK1811-B | Human CD164 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.079 | 0.180 | 0.266 | 0.410 | 0.667 | 1.083 | 1.610 | 2.099 |
Data Example Images
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Human CD164 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD164 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 105 | 366 | 847 | 124 | 456 | 964 |
| Standard deviation | 5.67 | 15.01 | 46.59 | 7.56 | 28.73 | 67.48 |
| CV(%) | 5.4% | 4.1% | 5.5% | 6.1% | 6.3% | 7.0% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD164 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 105 | 135 | 144 | 180 | 141 | 6.35 | 4.5% |
| Sample 2 | 366 | 370 | 285 | 294 | 329 | 16.77 | 5.1% |
| Sample 3 | 847 | 904 | 1006 | 1167 | 981 | 22.56 | 2.3% |
Protein Target Info & Infographic
Gene/Protein Information For CD164 (Source: Uniprot.Org, NCBI)
Gene Name
CD164
Full Name
Sialomucin core protein 24
Weight
20.917kDa
Superfamily
CD164 family
Alternative Names
CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin CD164 DFNA66, MGC-24, MGC-24v, MUC-24, endolyn CD164 molecule sialomucin core protein 24|CD164 , sialomucin|multi-glycosylated core protein 24
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD164, check out the CD164 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD164: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD164 PicoKine® Quick ELISA Kit (FEK1811)
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